CRISPR-U™ Gene Knockout Cell Line
Strategy
BATF2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BATF2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BATF2 |
| Gene id | 116071 |
| Organism | Homo sapiens |
| Official full symbol | basic leucine zipper ATF-like transcription factor 2 |
| Gene type | protein-coding |
| Also known as | SARI |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BATF2-201 | ENST00000301887.9 | 2066 | 274aa | Protein coding | CCDS8087 | Q8N1L9-1 | NM_138456.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BATF2-202 | ENST00000435842.2 | 1949 | 189aa | Protein coding | CCDS73317 | Q8N1L9-2 | - | TSL:1, GENCODE basic, |
| BATF2-204 | ENST00000527716.1 | 1230 | 250aa | Protein coding | CCDS76429 | B4DV37 | - | TSL:2, GENCODE basic, |
| BATF2-205 | ENST00000534177.1 | 569 | 155aa | Protein coding | - | E9PJ73 | - | CDS 3' incomplete, TSL:4, |
| BATF2-203 | ENST00000527454.1 | 2569 | No protein | Retained intron | - | - | - | TSL:NA, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BATF2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BATF2 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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