CRISPR-U™ Gene Knockout Cell Line
Strategy
GBP4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GBP4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GBP4 |
| Gene id | 115361 |
| Organism | Homo sapiens |
| Official full symbol | guanylate binding protein 4 |
| Gene type | protein-coding |
| Also known as | Mpa2 |
| Summary | Guanylate-binding proteins, such as GBP4, are induced by interferon and hydrolyze GTP to both GDP and GMP (Vestal, 2005 ). |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GBP4-201 | ENST00000355754.7 | 6141 | 640aa | Protein coding | CCDS721 | Q96PP9 | NM_052941.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GBP4-202 | ENST00000471938.1 | 768 | No protein | Processed transcript | - | - | - | TSL:3, |
| GBP4-203 | ENST00000481397.1 | 457 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GBP4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GBP4 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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