CRISPR-U™ Gene Knockout Cell Line
Strategy
CIRBP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CIRBP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CIRBP |
| Gene id | 1153 |
| Organism | Homo sapiens |
| Official full symbol | cold inducible RNA binding protein |
| Gene type | protein-coding |
| Also known as | CIRP |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 22 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CIRBP-214 | ENST00000587896.6 | 3346 | 297aa | Protein coding | CCDS74245 | D6W5Y5 | NM_001300829.2 | TSL:2, GENCODE basic, MANE Select v0.92, |
| CIRBP-225 | ENST00000589710.5 | 1835 | 297aa | Protein coding | CCDS74245 | D6W5Y5 | - | TSL:2, GENCODE basic, |
| CIRBP-215 | ENST00000588030.5 | 1509 | 172aa | Protein coding | CCDS12059 | Q14011-1 | - | TSL:5, GENCODE basic, APPRIS P3, |
| CIRBP-216 | ENST00000588090.5 | 1459 | 172aa | Protein coding | CCDS12059 | Q14011-1 | - | TSL:3, GENCODE basic, APPRIS P3, |
| CIRBP-201 | ENST00000320936.9 | 1328 | 172aa | Protein coding | CCDS12059 | Q14011-1 | - | TSL:1, GENCODE basic, APPRIS P3, |
| CIRBP-207 | ENST00000586472.5 | 1303 | 172aa | Protein coding | CCDS12059 | Q14011-1 | - | TSL:3, GENCODE basic, APPRIS P3, |
| CIRBP-213 | ENST00000587323.5 | 1092 | 297aa | Protein coding | CCDS74245 | D6W5Y5 | - | TSL:5, GENCODE basic, |
| CIRBP-222 | ENST00000589235.5 | 1073 | 168aa | Protein coding | CCDS74244 | K7EPM4 | - | TSL:2, GENCODE basic, APPRIS ALT1, |
| CIRBP-246 | ENST00000628979.2 | 1043 | 297aa | Protein coding | CCDS74245 | D6W5Y5 | - | TSL:2, GENCODE basic, |
| CIRBP-217 | ENST00000588230.5 | 1013 | 297aa | Protein coding | CCDS74245 | D6W5Y5 | - | TSL:5, GENCODE basic, |
| CIRBP-211 | ENST00000586773.5 | 942 | 168aa | Protein coding | CCDS74244 | K7EPM4 | - | TSL:2, GENCODE basic, APPRIS ALT1, |
| CIRBP-204 | ENST00000585630.5 | 870 | 172aa | Protein coding | CCDS12059 | Q14011-1 | - | TSL:3, GENCODE basic, APPRIS P3, |
| CIRBP-235 | ENST00000591935.5 | 746 | 168aa | Protein coding | CCDS74244 | K7EPM4 | - | TSL:3, GENCODE basic, APPRIS ALT1, |
| CIRBP-208 | ENST00000586548.5 | 1734 | 159aa | Protein coding | - | K7EMY9 | - | TSL:3, |
| CIRBP-202 | ENST00000413636.6 | 1606 | 263aa | Protein coding | - | Q14011-2 | - | TSL:2, GENCODE basic, |
| CIRBP-219 | ENST00000588411.5 | 619 | 105aa | Protein coding | - | K7EJV5 | - | CDS 3' incomplete, TSL:5, |
| CIRBP-223 | ENST00000589266.5 | 616 | 80aa | Protein coding | - | K7ENX8 | - | CDS 3' incomplete, TSL:3, |
| CIRBP-234 | ENST00000591659.5 | 587 | 173aa | Protein coding | - | K7ER40 | - | CDS 3' incomplete, TSL:2, |
| CIRBP-236 | ENST00000592051.1 | 573 | 32aa | Protein coding | - | K7EQX4 | - | CDS 3' incomplete, TSL:4, |
| CIRBP-224 | ENST00000589686.5 | 532 | 148aa | Protein coding | - | K7EJV1 | - | CDS 3' incomplete, TSL:2, |
| CIRBP-218 | ENST00000588344.2 | 370 | 89aa | Protein coding | - | K7ELV6 | - | CDS 5' incomplete, TSL:3, |
| CIRBP-240 | ENST00000592815.5 | 334 | 38aa | Protein coding | - | K7ENN6 | - | CDS 3' incomplete, TSL:4, |
| CIRBP-210 | ENST00000586636.5 | 938 | 168aa | Nonsense mediated decay | CCDS74244 | K7EPM4 | - | TSL:2, |
| CIRBP-205 | ENST00000585913.5 | 833 | 168aa | Nonsense mediated decay | CCDS74244 | K7EPM4 | - | TSL:3, |
| CIRBP-228 | ENST00000590347.5 | 3117 | 135aa | Nonsense mediated decay | - | K7ELT6 | - | TSL:2, |
| CIRBP-212 | ENST00000587169.5 | 3028 | 79aa | Nonsense mediated decay | - | K7EQL0 | - | TSL:2, |
| CIRBP-231 | ENST00000591097.5 | 1507 | 60aa | Nonsense mediated decay | - | K7EIF7 | - | TSL:1, |
| CIRBP-206 | ENST00000585914.5 | 1052 | 202aa | Nonsense mediated decay | - | K7EQR7 | - | TSL:5, |
| CIRBP-230 | ENST00000591055.5 | 928 | 135aa | Nonsense mediated decay | - | K7ELT6 | - | TSL:2, |
| CIRBP-226 | ENST00000590171.1 | 905 | 60aa | Nonsense mediated decay | - | K7EIF7 | - | TSL:3, |
| CIRBP-232 | ENST00000591376.5 | 830 | 79aa | Nonsense mediated decay | - | K7EQL0 | - | TSL:3, |
| CIRBP-241 | ENST00000593048.5 | 650 | 79aa | Nonsense mediated decay | - | K7EQL0 | - | TSL:3, |
| CIRBP-203 | ENST00000444172.6 | 584 | 58aa | Nonsense mediated decay | - | F6WMK9 | - | CDS 5' incomplete, TSL:3, |
| CIRBP-237 | ENST00000592234.5 | 571 | 73aa | Nonsense mediated decay | - | K7ELL4 | - | CDS 5' incomplete, TSL:5, |
| CIRBP-238 | ENST00000592412.5 | 532 | 79aa | Nonsense mediated decay | - | K7EQL0 | - | TSL:4, |
| CIRBP-227 | ENST00000590188.5 | 361 | No protein | Processed transcript | - | - | - | TSL:3, |
| CIRBP-242 | ENST00000593093.5 | 3528 | No protein | Retained intron | - | - | - | TSL:2, |
| CIRBP-229 | ENST00000590704.5 | 1456 | No protein | Retained intron | - | - | - | TSL:2, |
| CIRBP-220 | ENST00000588917.2 | 1087 | No protein | Retained intron | - | - | - | TSL:2, |
| CIRBP-245 | ENST00000621399.1 | 1044 | No protein | Retained intron | - | - | - | TSL:1, |
| CIRBP-239 | ENST00000592467.1 | 796 | No protein | Retained intron | - | - | - | TSL:2, |
| CIRBP-243 | ENST00000593128.5 | 796 | No protein | Retained intron | - | - | - | TSL:3, |
| CIRBP-221 | ENST00000589161.1 | 769 | No protein | Retained intron | - | - | - | TSL:3, |
| CIRBP-244 | ENST00000593283.5 | 758 | No protein | Retained intron | - | - | - | TSL:5, |
| CIRBP-209 | ENST00000586555.2 | 716 | No protein | Retained intron | - | - | - | TSL:2, |
| CIRBP-233 | ENST00000591622.1 | 548 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CIRBP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CIRBP had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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