CRISPR-U™ Gene Knockout Cell Line
Strategy
MYSM1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MYSM1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MYSM1 |
| Gene id | 114803 |
| Organism | Homo sapiens |
| Official full symbol | Myb like, SWIRM and MPN domains 1 |
| Gene type | protein-coding |
| Also known as | 2A-DUB, 2ADUB, BMFS4 |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MYSM1-202 | ENST00000472487.6 | 7751 | 828aa | Protein coding | CCDS41343 | Q5VVJ2-1 | NM_001085487.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| MYSM1-207 | ENST00000622766.1 | 6020 | 234aa | Protein coding | - | Q5VVJ2-3 | - | TSL:1, GENCODE basic, |
| MYSM1-208 | ENST00000655340.1 | 4436 | 806aa | Protein coding | - | A0A590UJD8 | - | GENCODE basic, APPRIS ALT2, |
| MYSM1-212 | ENST00000665648.1 | 7192 | 573aa | Nonsense mediated decay | - | A0A590UJW2 | - | - |
| MYSM1-209 | ENST00000659108.1 | 5354 | 111aa | Nonsense mediated decay | - | A0A590UJT8 | - | - |
| MYSM1-210 | ENST00000659812.1 | 3533 | 509aa | Nonsense mediated decay | - | A0A590UK32 | - | CDS 5' incomplete, |
| MYSM1-206 | ENST00000493821.6 | 7794 | No protein | Processed transcript | - | - | - | TSL:1, |
| MYSM1-211 | ENST00000660611.1 | 3306 | No protein | Processed transcript | - | - | - | - |
| MYSM1-201 | ENST00000401044.7 | 3176 | No protein | Processed transcript | - | - | - | TSL:1, |
| MYSM1-205 | ENST00000489282.1 | 698 | No protein | Processed transcript | - | - | - | TSL:2, |
| MYSM1-203 | ENST00000481973.1 | 397 | No protein | Processed transcript | - | - | - | TSL:3, |
| MYSM1-204 | ENST00000483003.5 | 378 | No protein | Processed transcript | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MYSM1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MYSM1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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