CRISPR-U™ Gene Knockout Cell Line Strategy

MYSM1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MYSM1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MYSM1
Gene id 114803
Organism Homo sapiens
Official full symbol Myb like, SWIRM and MPN domains 1
Gene type protein-coding
Also known as 2A-DUB, 2ADUB, BMFS4
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MYSM1-202 ENST00000472487.6 7751 828aa Protein coding CCDS41343 Q5VVJ2-1 NM_001085487.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
MYSM1-207 ENST00000622766.1 6020 234aa Protein coding - Q5VVJ2-3 - TSL:1, GENCODE basic,
MYSM1-208 ENST00000655340.1 4436 806aa Protein coding - A0A590UJD8 - GENCODE basic, APPRIS ALT2,
MYSM1-212 ENST00000665648.1 7192 573aa Nonsense mediated decay - A0A590UJW2 - -
MYSM1-209 ENST00000659108.1 5354 111aa Nonsense mediated decay - A0A590UJT8 - -
MYSM1-210 ENST00000659812.1 3533 509aa Nonsense mediated decay - A0A590UK32 - CDS 5' incomplete,
MYSM1-206 ENST00000493821.6 7794 No protein Processed transcript - - - TSL:1,
MYSM1-211 ENST00000660611.1 3306 No protein Processed transcript - - - -
MYSM1-201 ENST00000401044.7 3176 No protein Processed transcript - - - TSL:1,
MYSM1-205 ENST00000489282.1 698 No protein Processed transcript - - - TSL:2,
MYSM1-203 ENST00000481973.1 397 No protein Processed transcript - - - TSL:3,
MYSM1-204 ENST00000483003.5 378 No protein Processed transcript - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MYSM1
This KO Strategy loading
Red Cotton™ Notes Gene MYSM1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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