CRISPR-U™ Gene Knockout Cell Line
Strategy
C12orf57 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human C12orf57 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | C12orf57 |
| Gene id | 113246 |
| Organism | Homo sapiens |
| Official full symbol | chromosome 12 open reading frame 57 |
| Gene type | protein-coding |
| Also known as | C10, GRCC10 |
| Summary | This gene is ubiquitously expressed in human tissues. It is required for development of the human corpus callosum. Mutations in this gene are associated with Temtamy syndrome (TEMTYS). Multiple alternatively spliced transcript variants have been found for this gene. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| C12orf57-202 | ENST00000537087.5 | 662 | 97aa | Protein coding | CCDS76515 | F5GXW5 | - | TSL:2, GENCODE basic, |
| C12orf57-201 | ENST00000229281.6 | 561 | 126aa | Protein coding | CCDS8571 | Q99622 | NM_138425.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| C12orf57-204 | ENST00000540506.2 | 551 | 91aa | Protein coding | CCDS76516 | U3KQ85 | - | TSL:2, GENCODE basic, |
| C12orf57-206 | ENST00000544681.1 | 980 | 103aa | Protein coding | - | U3KQ07 | - | TSL:2, GENCODE basic, |
| C12orf57-207 | ENST00000545581.5 | 556 | 126aa | Protein coding | - | Q99622 | - | CDS 3' incomplete, TSL:3, |
| C12orf57-205 | ENST00000542222.1 | 640 | No protein | Processed transcript | - | - | - | TSL:2, |
| C12orf57-203 | ENST00000538392.1 | 979 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | C12orf57 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene C12orf57 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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