CRISPR-U™ Gene Knockout Cell Line Strategy

C12orf57 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human C12orf57 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol C12orf57
Gene id 113246
Organism Homo sapiens
Official full symbol chromosome 12 open reading frame 57
Gene type protein-coding
Also known as C10, GRCC10
Summary This gene is ubiquitously expressed in human tissues. It is required for development of the human corpus callosum. Mutations in this gene are associated with Temtamy syndrome (TEMTYS). Multiple alternatively spliced transcript variants have been found for this gene.
Genomic regions Chromosome 12
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
C12orf57-202 ENST00000537087.5 662 97aa Protein coding CCDS76515 F5GXW5 - TSL:2, GENCODE basic,
C12orf57-201 ENST00000229281.6 561 126aa Protein coding CCDS8571 Q99622 NM_138425.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
C12orf57-204 ENST00000540506.2 551 91aa Protein coding CCDS76516 U3KQ85 - TSL:2, GENCODE basic,
C12orf57-206 ENST00000544681.1 980 103aa Protein coding - U3KQ07 - TSL:2, GENCODE basic,
C12orf57-207 ENST00000545581.5 556 126aa Protein coding - Q99622 - CDS 3' incomplete, TSL:3,
C12orf57-205 ENST00000542222.1 640 No protein Processed transcript - - - TSL:2,
C12orf57-203 ENST00000538392.1 979 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene C12orf57
This KO Strategy loading
Red Cotton™ Notes Gene C12orf57 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×