CRISPR-U™ Gene Knockout Cell Line Strategy

PARK7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PARK7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PARK7
Gene id 11315
Organism Homo sapiens
Official full symbol Parkinsonism associated deglycase
Gene type protein-coding
Also known as DJ-1, DJ1, GATD2, HEL-S-67p
Summary The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PARK7-201 ENST00000338639.10 1127 189aa Protein coding CCDS93 Q99497 V9HWC2 NM_007262.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PARK7-209 ENST00000493678.5 1088 189aa Protein coding CCDS93 Q99497 V9HWC2 - TSL:1, GENCODE basic, APPRIS P1,
PARK7-203 ENST00000377491.5 977 189aa Protein coding CCDS93 Q99497 V9HWC2 - TSL:3, GENCODE basic, APPRIS P1,
PARK7-202 ENST00000377488.5 783 189aa Protein coding CCDS93 Q99497 V9HWC2 - TSL:3, GENCODE basic, APPRIS P1,
PARK7-204 ENST00000377493.9 795 169aa Protein coding - K7ELW0 - TSL:2, GENCODE basic,
PARK7-207 ENST00000469225.1 711 160aa Protein coding - K7EN27 - CDS 5' incomplete, TSL:3,
PARK7-208 ENST00000493373.5 624 189aa Protein coding - Q99497 V9HWC2 - CDS 3' incomplete, TSL:5,
PARK7-210 ENST00000497113.1 670 No protein Processed transcript - - - TSL:3,
PARK7-206 ENST00000465354.5 949 No protein Retained intron - - - TSL:2,
PARK7-205 ENST00000460192.5 567 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PARK7
This KO Strategy loading
Red Cotton™ Notes Gene PARK7 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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