CRISPR-U™ Gene Knockout Cell Line
Strategy
PARK7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PARK7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PARK7 |
| Gene id | 11315 |
| Organism | Homo sapiens |
| Official full symbol | Parkinsonism associated deglycase |
| Gene type | protein-coding |
| Also known as | DJ-1, DJ1, GATD2, HEL-S-67p |
| Summary | The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PARK7-201 | ENST00000338639.10 | 1127 | 189aa | Protein coding | CCDS93 | Q99497 V9HWC2 | NM_007262.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PARK7-209 | ENST00000493678.5 | 1088 | 189aa | Protein coding | CCDS93 | Q99497 V9HWC2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PARK7-203 | ENST00000377491.5 | 977 | 189aa | Protein coding | CCDS93 | Q99497 V9HWC2 | - | TSL:3, GENCODE basic, APPRIS P1, |
| PARK7-202 | ENST00000377488.5 | 783 | 189aa | Protein coding | CCDS93 | Q99497 V9HWC2 | - | TSL:3, GENCODE basic, APPRIS P1, |
| PARK7-204 | ENST00000377493.9 | 795 | 169aa | Protein coding | - | K7ELW0 | - | TSL:2, GENCODE basic, |
| PARK7-207 | ENST00000469225.1 | 711 | 160aa | Protein coding | - | K7EN27 | - | CDS 5' incomplete, TSL:3, |
| PARK7-208 | ENST00000493373.5 | 624 | 189aa | Protein coding | - | Q99497 V9HWC2 | - | CDS 3' incomplete, TSL:5, |
| PARK7-210 | ENST00000497113.1 | 670 | No protein | Processed transcript | - | - | - | TSL:3, |
| PARK7-206 | ENST00000465354.5 | 949 | No protein | Retained intron | - | - | - | TSL:2, |
| PARK7-205 | ENST00000460192.5 | 567 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PARK7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PARK7 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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