CRISPR-U™ Gene Knockout Cell Line Strategy

LYPLA2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LYPLA2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LYPLA2
Gene id 11313
Organism Homo sapiens
Official full symbol lysophospholipase 2
Gene type protein-coding
Also known as APT-2, APT2, DJ886K2.4
Summary Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet.
Genomic regions Chromosome 1
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LYPLA2-205 ENST00000374514.8 1635 231aa Protein coding CCDS241 A0A140VJC9 O95372 NM_007260.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LYPLA2-201 ENST00000374501.1 1117 164aa Protein coding - Q5QPQ0 - TSL:2, GENCODE basic,
LYPLA2-202 ENST00000374502.7 1072 180aa Protein coding - Q5QPQ3 - TSL:2, GENCODE basic,
LYPLA2-204 ENST00000374505.6 907 137aa Protein coding - Q5QPN9 - TSL:3, GENCODE basic,
LYPLA2-203 ENST00000374503.7 868 182aa Protein coding - Q5QPQ2 - TSL:2, GENCODE basic,
LYPLA2-206 ENST00000420982.5 614 162aa Protein coding - Q5QPQ1 - CDS 3' incomplete, TSL:3,
LYPLA2-207 ENST00000421070.1 429 90aa Protein coding - Q5QPN5 - CDS 3' incomplete, TSL:5,
LYPLA2-210 ENST00000495365.5 842 No protein Processed transcript - - - TSL:2,
LYPLA2-208 ENST00000472213.1 822 No protein Processed transcript - - - TSL:3,
LYPLA2-209 ENST00000492577.1 717 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LYPLA2
This KO Strategy loading
Red Cotton™ Notes Gene LYPLA2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×