CRISPR-U™ Gene Knockout Cell Line
Strategy
LYPLA2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LYPLA2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LYPLA2 |
| Gene id | 11313 |
| Organism | Homo sapiens |
| Official full symbol | lysophospholipase 2 |
| Gene type | protein-coding |
| Also known as | APT-2, APT2, DJ886K2.4 |
| Summary | Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LYPLA2-205 | ENST00000374514.8 | 1635 | 231aa | Protein coding | CCDS241 | A0A140VJC9 O95372 | NM_007260.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LYPLA2-201 | ENST00000374501.1 | 1117 | 164aa | Protein coding | - | Q5QPQ0 | - | TSL:2, GENCODE basic, |
| LYPLA2-202 | ENST00000374502.7 | 1072 | 180aa | Protein coding | - | Q5QPQ3 | - | TSL:2, GENCODE basic, |
| LYPLA2-204 | ENST00000374505.6 | 907 | 137aa | Protein coding | - | Q5QPN9 | - | TSL:3, GENCODE basic, |
| LYPLA2-203 | ENST00000374503.7 | 868 | 182aa | Protein coding | - | Q5QPQ2 | - | TSL:2, GENCODE basic, |
| LYPLA2-206 | ENST00000420982.5 | 614 | 162aa | Protein coding | - | Q5QPQ1 | - | CDS 3' incomplete, TSL:3, |
| LYPLA2-207 | ENST00000421070.1 | 429 | 90aa | Protein coding | - | Q5QPN5 | - | CDS 3' incomplete, TSL:5, |
| LYPLA2-210 | ENST00000495365.5 | 842 | No protein | Processed transcript | - | - | - | TSL:2, |
| LYPLA2-208 | ENST00000472213.1 | 822 | No protein | Processed transcript | - | - | - | TSL:3, |
| LYPLA2-209 | ENST00000492577.1 | 717 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LYPLA2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LYPLA2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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