CRISPR-U™ Gene Knockout Cell Line Strategy

ATXN2L Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ATXN2L Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATXN2L
Gene id 11273
Organism Homo sapiens
Official full symbol ataxin 2 like
Gene type protein-coding
Also known as A2D, A2LG, A2LP, A2RP
Summary This gene encodes an ataxin type 2 related protein of unknown function. This protein is a member of the spinocerebellar ataxia (SCAs) family, which is associated with a complex group of neurodegenerative disorders. Several alternatively spliced transcripts encoding different isoforms have been found for this gene.
Genomic regions Chromosome 16
Strategy Summary
This gene has 14 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATXN2L-202 ENST00000336783.8 4367 1075aa Protein coding CCDS10641 Q8WWM7-1 - TSL:1, GENCODE basic, APPRIS P3,
ATXN2L-205 ENST00000395547.6 3981 1097aa Protein coding CCDS10640 Q8WWM7-3 - TSL:1, GENCODE basic, APPRIS ALT2,
ATXN2L-203 ENST00000340394.12 3807 1044aa Protein coding CCDS10639 Q8WWM7-4 - TSL:1, GENCODE basic, APPRIS ALT2,
ATXN2L-201 ENST00000325215.10 3788 1062aa Protein coding CCDS32423 Q8WWM7-2 - TSL:1, GENCODE basic, APPRIS ALT2,
ATXN2L-204 ENST00000382686.8 3739 1044aa Protein coding CCDS45451 Q8WWM7-8 - TSL:1, GENCODE basic, APPRIS ALT2,
ATXN2L-225 ENST00000570200.5 3635 1062aa Protein coding CCDS58443 Q8WWM7-9 - TSL:1, GENCODE basic, APPRIS ALT2,
ATXN2L-215 ENST00000564304.5 3514 1068aa Protein coding CCDS76850 H3BUF6 - TSL:1, GENCODE basic, APPRIS ALT2,
ATXN2L-221 ENST00000566946.5 2162 401aa Protein coding - A0A0C4DGL7 - CDS 5' incomplete, TSL:1,
ATXN2L-223 ENST00000568266.5 1294 336aa Protein coding - H3BSK9 - CDS 3' incomplete, TSL:5,
ATXN2L-213 ENST00000564162.1 770 110aa Protein coding - H3BPQ7 - CDS 5' incomplete, TSL:5,
ATXN2L-224 ENST00000569318.1 559 187aa Protein coding - H3BMX1 - CDS 5' and 3' incomplete, TSL:5,
ATXN2L-216 ENST00000564656.5 552 91aa Protein coding - H3BSQ5 - CDS 3' incomplete, TSL:4,
ATXN2L-222 ENST00000567024.1 525 11aa Protein coding - H3BQ14 - CDS 5' incomplete, TSL:3,
ATXN2L-219 ENST00000566007.5 471 128aa Protein coding - H3BQX7 - CDS 5' incomplete, TSL:3,
ATXN2L-218 ENST00000565971.5 3264 217aa Nonsense mediated decay - H3BUE3 - CDS 5' incomplete, TSL:1,
ATXN2L-208 ENST00000562583.5 1933 400aa Nonsense mediated decay - H3BRB0 - CDS 5' incomplete, TSL:1,
ATXN2L-217 ENST00000565845.5 750 No protein Processed transcript - - - TSL:5,
ATXN2L-226 ENST00000570284.1 701 No protein Processed transcript - - - TSL:5,
ATXN2L-211 ENST00000563314.5 4574 No protein Retained intron - - - TSL:5,
ATXN2L-212 ENST00000564035.5 786 No protein Retained intron - - - TSL:2,
ATXN2L-207 ENST00000561732.1 643 No protein Retained intron - - - TSL:2,
ATXN2L-210 ENST00000562867.5 609 No protein Retained intron - - - TSL:2,
ATXN2L-214 ENST00000564284.1 603 No protein Retained intron - - - TSL:2,
ATXN2L-206 ENST00000561539.1 509 No protein Retained intron - - - TSL:3,
ATXN2L-209 ENST00000562686.1 489 No protein Retained intron - - - TSL:3,
ATXN2L-220 ENST00000566080.1 329 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATXN2L
This KO Strategy loading
Red Cotton™ Notes Gene ATXN2L had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×