CRISPR-U™ Gene Knockout Cell Line
Strategy
ATXN2L Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATXN2L Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ATXN2L |
| Gene id | 11273 |
| Organism | Homo sapiens |
| Official full symbol | ataxin 2 like |
| Gene type | protein-coding |
| Also known as | A2D, A2LG, A2LP, A2RP |
| Summary | This gene encodes an ataxin type 2 related protein of unknown function. This protein is a member of the spinocerebellar ataxia (SCAs) family, which is associated with a complex group of neurodegenerative disorders. Several alternatively spliced transcripts encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 14 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ATXN2L-202 | ENST00000336783.8 | 4367 | 1075aa | Protein coding | CCDS10641 | Q8WWM7-1 | - | TSL:1, GENCODE basic, APPRIS P3, |
| ATXN2L-205 | ENST00000395547.6 | 3981 | 1097aa | Protein coding | CCDS10640 | Q8WWM7-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ATXN2L-203 | ENST00000340394.12 | 3807 | 1044aa | Protein coding | CCDS10639 | Q8WWM7-4 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ATXN2L-201 | ENST00000325215.10 | 3788 | 1062aa | Protein coding | CCDS32423 | Q8WWM7-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ATXN2L-204 | ENST00000382686.8 | 3739 | 1044aa | Protein coding | CCDS45451 | Q8WWM7-8 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ATXN2L-225 | ENST00000570200.5 | 3635 | 1062aa | Protein coding | CCDS58443 | Q8WWM7-9 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ATXN2L-215 | ENST00000564304.5 | 3514 | 1068aa | Protein coding | CCDS76850 | H3BUF6 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ATXN2L-221 | ENST00000566946.5 | 2162 | 401aa | Protein coding | - | A0A0C4DGL7 | - | CDS 5' incomplete, TSL:1, |
| ATXN2L-223 | ENST00000568266.5 | 1294 | 336aa | Protein coding | - | H3BSK9 | - | CDS 3' incomplete, TSL:5, |
| ATXN2L-213 | ENST00000564162.1 | 770 | 110aa | Protein coding | - | H3BPQ7 | - | CDS 5' incomplete, TSL:5, |
| ATXN2L-224 | ENST00000569318.1 | 559 | 187aa | Protein coding | - | H3BMX1 | - | CDS 5' and 3' incomplete, TSL:5, |
| ATXN2L-216 | ENST00000564656.5 | 552 | 91aa | Protein coding | - | H3BSQ5 | - | CDS 3' incomplete, TSL:4, |
| ATXN2L-222 | ENST00000567024.1 | 525 | 11aa | Protein coding | - | H3BQ14 | - | CDS 5' incomplete, TSL:3, |
| ATXN2L-219 | ENST00000566007.5 | 471 | 128aa | Protein coding | - | H3BQX7 | - | CDS 5' incomplete, TSL:3, |
| ATXN2L-218 | ENST00000565971.5 | 3264 | 217aa | Nonsense mediated decay | - | H3BUE3 | - | CDS 5' incomplete, TSL:1, |
| ATXN2L-208 | ENST00000562583.5 | 1933 | 400aa | Nonsense mediated decay | - | H3BRB0 | - | CDS 5' incomplete, TSL:1, |
| ATXN2L-217 | ENST00000565845.5 | 750 | No protein | Processed transcript | - | - | - | TSL:5, |
| ATXN2L-226 | ENST00000570284.1 | 701 | No protein | Processed transcript | - | - | - | TSL:5, |
| ATXN2L-211 | ENST00000563314.5 | 4574 | No protein | Retained intron | - | - | - | TSL:5, |
| ATXN2L-212 | ENST00000564035.5 | 786 | No protein | Retained intron | - | - | - | TSL:2, |
| ATXN2L-207 | ENST00000561732.1 | 643 | No protein | Retained intron | - | - | - | TSL:2, |
| ATXN2L-210 | ENST00000562867.5 | 609 | No protein | Retained intron | - | - | - | TSL:2, |
| ATXN2L-214 | ENST00000564284.1 | 603 | No protein | Retained intron | - | - | - | TSL:2, |
| ATXN2L-206 | ENST00000561539.1 | 509 | No protein | Retained intron | - | - | - | TSL:3, |
| ATXN2L-209 | ENST00000562686.1 | 489 | No protein | Retained intron | - | - | - | TSL:3, |
| ATXN2L-220 | ENST00000566080.1 | 329 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ATXN2L |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ATXN2L had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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