CRISPR-U™ Gene Knockout Cell Line
Strategy
PACSIN2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PACSIN2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PACSIN2 |
| Gene id | 11252 |
| Organism | Homo sapiens |
| Official full symbol | protein kinase C and casein kinase substrate in neurons 2 |
| Gene type | protein-coding |
| Also known as | SDPII |
| Summary | This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PACSIN2-201 | ENST00000263246.8 | 3251 | 486aa | Protein coding | CCDS43023 | Q9UNF0-1 | NM_001184970.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PACSIN2-202 | ENST00000337959.8 | 3149 | 445aa | Protein coding | CCDS54536 | Q9UNF0-2 | - | TSL:5, GENCODE basic, |
| PACSIN2-205 | ENST00000407585.5 | 3080 | 445aa | Protein coding | CCDS54536 | Q9UNF0-2 | - | TSL:1, GENCODE basic, |
| PACSIN2-204 | ENST00000403744.7 | 2110 | 486aa | Protein coding | CCDS43023 | Q9UNF0-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PACSIN2-203 | ENST00000402229.5 | 1883 | 486aa | Protein coding | CCDS43023 | Q9UNF0-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| PACSIN2-212 | ENST00000634914.1 | 1431 | 451aa | Protein coding | - | A0A0U1RR22 | - | CDS 3' incomplete, TSL:5, |
| PACSIN2-210 | ENST00000453643.5 | 818 | 155aa | Protein coding | - | B0QYG8 | - | CDS 3' incomplete, TSL:5, |
| PACSIN2-207 | ENST00000422336.5 | 664 | 152aa | Protein coding | - | B0QYG7 | - | CDS 3' incomplete, TSL:5, |
| PACSIN2-206 | ENST00000418133.5 | 622 | 152aa | Protein coding | - | B0QYG7 | - | CDS 3' incomplete, TSL:5, |
| PACSIN2-209 | ENST00000453079.1 | 505 | 33aa | Protein coding | - | B0QYG9 | - | CDS 3' incomplete, TSL:2, |
| PACSIN2-211 | ENST00000507586.1 | 362 | 59aa | Nonsense mediated decay | - | H0Y923 | - | CDS 5' incomplete, TSL:3, |
| PACSIN2-208 | ENST00000445706.5 | 511 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PACSIN2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PACSIN2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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