CRISPR-U™ Gene Knockout Cell Line Strategy

PRRT2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PRRT2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PRRT2
Gene id 112476
Organism Homo sapiens
Official full symbol proline rich transmembrane protein 2
Gene type protein-coding
Also known as BFIC2, BFIS2, DSPB3, DYT10, EKD1, FICCA, ICCA, IFITMD1, PKC
Summary This gene encodes a transmembrane protein containing a proline-rich domain in its N-terminal half. Studies in mice suggest that it is predominantly expressed in brain and spinal cord in embryonic and postnatal stages. Mutations in this gene are associated with episodic kinesigenic dyskinesia-1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 16
Strategy Summary
This gene has 14 protein coding transcripts：
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PRRT2-201 ENST00000300797.7 3002 299aa Protein coding CCDS58446 Q7Z6L0-3 - TSL:2, GENCODE basic,
PRRT2-221 ENST00000647876.1 2867 299aa Protein coding CCDS58446 Q7Z6L0-3 - GENCODE basic,
PRRT2-202 ENST00000358758.12 2462 340aa Protein coding CCDS10654 Q7Z6L0-1 NM_145239.3 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
PRRT2-207 ENST00000572820.2 2403 340aa Protein coding CCDS10654 Q7Z6L0-1 - TSL:5, GENCODE basic, APPRIS ALT2,
PRRT2-214 ENST00000637064.1 2153 340aa Protein coding CCDS10654 Q7Z6L0-1 - TSL:5, GENCODE basic, APPRIS ALT2,
PRRT2-205 ENST00000567659.3 1470 394aa Protein coding CCDS58445 Q7Z6L0-2 - TSL:2, GENCODE basic, APPRIS P4,
PRRT2-212 ENST00000636619.1 2065 287aa Protein coding - A0A1B0GTE9 - TSL:5, GENCODE basic,
PRRT2-216 ENST00000637403.1 2033 286aa Protein coding - A0A1B0GTP1 - TSL:5, GENCODE basic,
PRRT2-219 ENST00000637565.1 1733 155aa Protein coding - A0A1B0GUR0 - TSL:5, GENCODE basic,
PRRT2-204 ENST00000567551.2 644 145aa Protein coding - A0A1B0GTS0 - CDS 3' incomplete, TSL:5,
PRRT2-203 ENST00000562148.2 564 106aa Protein coding - H3BN10 - CDS 3' incomplete, TSL:3,
PRRT2-220 ENST00000637596.1 520 128aa Protein coding - A0A1B0GTR2 - CDS 3' incomplete, TSL:5,
PRRT2-211 ENST00000636246.1 517 118aa Protein coding - A0A1B0GU37 - CDS 3' incomplete, TSL:5,
PRRT2-213 ENST00000636902.1 100 33aa Protein coding - A0A1B0GU25 - CDS 5' and 3' incomplete, TSL:5,
PRRT2-210 ENST00000636131.1 2365 299aa Nonsense mediated decay CCDS58446 Q7Z6L0-3 - TSL:5,
PRRT2-215 ENST00000637290.1 2009 130aa Nonsense mediated decay - A0A1B0GUW9 - TSL:5,
PRRT2-206 ENST00000568516.1 534 No protein Processed transcript - - - TSL:4,
PRRT2-209 ENST00000636019.1 131 No protein Processed transcript - - - TSL:5,
PRRT2-208 ENST00000636001.1 100 No protein Processed transcript - - - TSL:5,
PRRT2-217 ENST00000637425.1 100 No protein Processed transcript - - - TSL:5,
PRRT2-218 ENST00000637542.1 100 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PRRT2
This KO Strategy loading
Red Cotton™ Notes Gene PRRT2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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