CRISPR-U™ Gene Knockout Cell Line Strategy

PDCD10 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PDCD10 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PDCD10
Gene id 11235
Organism Homo sapiens
Official full symbol programmed cell death 10
Gene type protein-coding
Also known as CCM3, TFAR15
Summary This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Genomic regions Chromosome 3
Strategy Summary
This gene has 13 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PDCD10-201 ENST00000392750.7 2133 212aa Protein coding CCDS3202 Q9BUL8 NM_007217.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PDCD10-208 ENST00000473645.6 1360 212aa Protein coding CCDS3202 Q9BUL8 - TSL:1, GENCODE basic, APPRIS P1,
PDCD10-218 ENST00000497056.6 1276 212aa Protein coding CCDS3202 Q9BUL8 - TSL:1, GENCODE basic, APPRIS P1,
PDCD10-206 ENST00000470131.5 1026 212aa Protein coding CCDS3202 Q9BUL8 - TSL:5, GENCODE basic, APPRIS P1,
PDCD10-202 ENST00000461494.5 934 212aa Protein coding CCDS3202 Q9BUL8 - TSL:3, GENCODE basic, APPRIS P1,
PDCD10-209 ENST00000475915.6 849 202aa Protein coding - C9J5C3 - CDS 3' incomplete, TSL:5,
PDCD10-216 ENST00000492396.5 814 149aa Protein coding - C9J363 - TSL:5, GENCODE basic,
PDCD10-207 ENST00000471885.5 764 186aa Protein coding - C9J6F3 - TSL:5, GENCODE basic,
PDCD10-214 ENST00000487947.6 716 186aa Protein coding - C9J6F3 - TSL:5, GENCODE basic,
PDCD10-203 ENST00000462725.6 715 146aa Protein coding - C9JND6 - CDS 3' incomplete, TSL:5,
PDCD10-215 ENST00000492139.5 686 134aa Protein coding - C9JSA3 - CDS 3' incomplete, TSL:5,
PDCD10-205 ENST00000464360.5 585 79aa Protein coding - C9J932 - CDS 3' incomplete, TSL:3,
PDCD10-210 ENST00000479121.5 430 122aa Protein coding - H7C5M9 - CDS 5' incomplete, TSL:5,
PDCD10-217 ENST00000494502.6 757 59aa Nonsense mediated decay - F8WDF3 - TSL:5,
PDCD10-213 ENST00000487678.1 416 No protein Processed transcript - - - TSL:3,
PDCD10-204 ENST00000462830.1 225 No protein Processed transcript - - - TSL:3,
PDCD10-212 ENST00000483451.5 1119 No protein Retained intron - - - TSL:5,
PDCD10-211 ENST00000481136.1 674 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PDCD10
This KO Strategy loading
Red Cotton™ Notes Gene PDCD10 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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