CRISPR-U™ Gene Knockout Cell Line Strategy

NUDT5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NUDT5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NUDT5
Gene id 11164
Organism Homo sapiens
Official full symbol nudix hydrolase 5
Gene type protein-coding
Also known as YSA1, YSA1H, YSAH1, hNUDT5
Summary This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors.
Genomic regions Chromosome 10
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NUDT5-207 ENST00000491614.6 3195 219aa Protein coding CCDS7089 Q9UKK9 NM_014142.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NUDT5-201 ENST00000378927.7 1258 180aa Protein coding - A6NCQ0 - TSL:2, GENCODE basic,
NUDT5-209 ENST00000537776.5 1201 219aa Protein coding - Q9UKK9 - TSL:5, GENCODE basic, APPRIS P1,
NUDT5-203 ENST00000378940.7 1126 186aa Protein coding - A6NJU6 - TSL:5, GENCODE basic,
NUDT5-202 ENST00000378937.7 919 232aa Protein coding - A6NFX8 - TSL:3, GENCODE basic,
NUDT5-205 ENST00000444732.1 410 97aa Protein coding - C9JYY9 - CDS 3' incomplete, TSL:3,
NUDT5-206 ENST00000476462.5 622 137aa Nonsense mediated decay - H0YEY4 - CDS 5' incomplete, TSL:2,
NUDT5-204 ENST00000378952.7 917 No protein Processed transcript - - - TSL:5,
NUDT5-208 ENST00000498825.1 473 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NUDT5
This KO Strategy loading
Red Cotton™ Notes Gene NUDT5 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×