CRISPR-U™ Gene Knockout Cell Line
Strategy
NUDT5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NUDT5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NUDT5 |
| Gene id | 11164 |
| Organism | Homo sapiens |
| Official full symbol | nudix hydrolase 5 |
| Gene type | protein-coding |
| Also known as | YSA1, YSA1H, YSAH1, hNUDT5 |
| Summary | This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NUDT5-207 | ENST00000491614.6 | 3195 | 219aa | Protein coding | CCDS7089 | Q9UKK9 | NM_014142.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NUDT5-201 | ENST00000378927.7 | 1258 | 180aa | Protein coding | - | A6NCQ0 | - | TSL:2, GENCODE basic, |
| NUDT5-209 | ENST00000537776.5 | 1201 | 219aa | Protein coding | - | Q9UKK9 | - | TSL:5, GENCODE basic, APPRIS P1, |
| NUDT5-203 | ENST00000378940.7 | 1126 | 186aa | Protein coding | - | A6NJU6 | - | TSL:5, GENCODE basic, |
| NUDT5-202 | ENST00000378937.7 | 919 | 232aa | Protein coding | - | A6NFX8 | - | TSL:3, GENCODE basic, |
| NUDT5-205 | ENST00000444732.1 | 410 | 97aa | Protein coding | - | C9JYY9 | - | CDS 3' incomplete, TSL:3, |
| NUDT5-206 | ENST00000476462.5 | 622 | 137aa | Nonsense mediated decay | - | H0YEY4 | - | CDS 5' incomplete, TSL:2, |
| NUDT5-204 | ENST00000378952.7 | 917 | No protein | Processed transcript | - | - | - | TSL:5, |
| NUDT5-208 | ENST00000498825.1 | 473 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NUDT5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NUDT5 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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