CRISPR-U™ Gene Knockout Cell Line Strategy

CD160 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CD160 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CD160
Gene id 11126
Organism Homo sapiens
Official full symbol CD160 molecule
Gene type protein-coding
Also known as BY55, NK1, NK28
Summary CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules.
Genomic regions Chromosome 1
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CD160-202 ENST00000369288.7 1580 181aa Protein coding CCDS72861 O95971-1 NM_007053.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CD160-201 ENST00000235933.10 1425 181aa Protein coding CCDS72861 O95971-1 - TSL:5, GENCODE basic, APPRIS P4,
CD160-205 ENST00000616463.1 3388 125aa Protein coding - O95971-4 - TSL:2, GENCODE basic, APPRIS ALT2,
CD160-203 ENST00000401557.7 705 234aa Protein coding - O95971-3 - TSL:1, GENCODE basic, APPRIS ALT2,
CD160-204 ENST00000584442.2 219 72aa Protein coding - O95971-2 - TSL:5, GENCODE basic, APPRIS ALT2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CD160
This KO Strategy loading
Red Cotton™ Notes Gene CD160 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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