CRISPR-U™ Gene Knockout Cell Line
Strategy
CD160 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CD160 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CD160 |
| Gene id | 11126 |
| Organism | Homo sapiens |
| Official full symbol | CD160 molecule |
| Gene type | protein-coding |
| Also known as | BY55, NK1, NK28 |
| Summary | CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CD160-202 | ENST00000369288.7 | 1580 | 181aa | Protein coding | CCDS72861 | O95971-1 | NM_007053.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| CD160-201 | ENST00000235933.10 | 1425 | 181aa | Protein coding | CCDS72861 | O95971-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| CD160-205 | ENST00000616463.1 | 3388 | 125aa | Protein coding | - | O95971-4 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| CD160-203 | ENST00000401557.7 | 705 | 234aa | Protein coding | - | O95971-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| CD160-204 | ENST00000584442.2 | 219 | 72aa | Protein coding | - | O95971-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CD160 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CD160 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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