CRISPR-U™ Gene Knockout Cell Line
Strategy
HNRNPUL1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HNRNPUL1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HNRNPUL1 |
| Gene id | 11100 |
| Organism | Homo sapiens |
| Official full symbol | heterogeneous nuclear ribonucleoprotein U like 1 |
| Gene type | protein-coding |
| Also known as | E1B-AP5, E1BAP5, HNRPUL1 |
| Summary | This gene encodes a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. This protein binds specifically to adenovirus early-1B-55kDa oncoprotein. It may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by early-1B-55kDa in adenovirus-infected cells. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 15 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HNRNPUL1-204 | ENST00000392006.8 | 3925 | 856aa | Protein coding | CCDS12576 | Q9BUJ2-1 | NM_007040.6 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| HNRNPUL1-201 | ENST00000263367.7 | 2952 | 767aa | Protein coding | CCDS82351 | B7Z4B8 | - | TSL:2, GENCODE basic, |
| HNRNPUL1-207 | ENST00000595018.5 | 2794 | 756aa | Protein coding | CCDS12577 | Q9BUJ2-4 | - | TSL:2, GENCODE basic, |
| HNRNPUL1-205 | ENST00000593587.5 | 2725 | 756aa | Protein coding | CCDS12577 | Q9BUJ2-4 | - | TSL:2, GENCODE basic, |
| HNRNPUL1-202 | ENST00000352456.7 | 3426 | 766aa | Protein coding | - | A0A0A0MRA5 | - | TSL:1, GENCODE basic, |
| HNRNPUL1-220 | ENST00000602130.5 | 2932 | 804aa | Protein coding | - | Q9BUJ2-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| HNRNPUL1-203 | ENST00000378215.8 | 2864 | 752aa | Protein coding | - | Q9BUJ2-3 | - | TSL:2, GENCODE basic, |
| HNRNPUL1-213 | ENST00000599614.5 | 2447 | 641aa | Protein coding | - | M0R3F1 | - | CDS 5' incomplete, TSL:5, |
| HNRNPUL1-214 | ENST00000599719.5 | 1473 | 371aa | Protein coding | - | M0QYZ0 | - | CDS 3' incomplete, TSL:1, |
| HNRNPUL1-221 | ENST00000617305.4 | 1018 | 122aa | Protein coding | - | A0A087X1I2 | - | TSL:1, GENCODE basic, |
| HNRNPUL1-219 | ENST00000601336.5 | 754 | 158aa | Protein coding | - | M0R0K8 | - | CDS 3' incomplete, TSL:2, |
| HNRNPUL1-218 | ENST00000601309.5 | 672 | 118aa | Protein coding | - | M0QZV6 | - | CDS 3' incomplete, TSL:5, |
| HNRNPUL1-211 | ENST00000597725.5 | 573 | 172aa | Protein coding | - | M0QYI8 | - | CDS 3' incomplete, TSL:4, |
| HNRNPUL1-222 | ENST00000617774.1 | 451 | 122aa | Protein coding | - | A0A087X1I2 | - | TSL:5, GENCODE basic, |
| HNRNPUL1-209 | ENST00000595336.1 | 393 | 81aa | Protein coding | - | M0R203 | - | CDS 3' incomplete, TSL:2, |
| HNRNPUL1-208 | ENST00000595196.5 | 3043 | 105aa | Nonsense mediated decay | - | M0R247 | - | TSL:1, |
| HNRNPUL1-215 | ENST00000600332.1 | 644 | 26aa | Nonsense mediated decay | - | M0QYG9 | - | CDS 5' incomplete, TSL:3, |
| HNRNPUL1-217 | ENST00000600596.1 | 581 | 90aa | Nonsense mediated decay | - | M0QYM5 | - | CDS 5' incomplete, TSL:5, |
| HNRNPUL1-206 | ENST00000594207.1 | 816 | No protein | Processed transcript | - | - | - | TSL:5, |
| HNRNPUL1-216 | ENST00000600493.1 | 531 | No protein | Processed transcript | - | - | - | TSL:4, |
| HNRNPUL1-210 | ENST00000595806.1 | 1659 | No protein | Retained intron | - | - | - | TSL:2, |
| HNRNPUL1-212 | ENST00000599521.1 | 336 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HNRNPUL1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HNRNPUL1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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