CRISPR-U™ Gene Knockout Cell Line
Strategy
DNAJB4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DNAJB4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DNAJB4 |
| Gene id | 11080 |
| Organism | Homo sapiens |
| Official full symbol | DnaJ heat shock protein family (Hsp40) member B4 |
| Gene type | protein-coding |
| Also known as | DNAJW, DjB4, HLJ1 |
| Summary | The protein encoded by this gene is a molecular chaperone, tumor suppressor, and member of the heat shock protein-40 family. The encoded protein binds the cell adhesion protein E-cadherin and targets it to the plasma membrane. This protein also binds incorrectly folded E-cadherin and targets it for endoplasmic reticulum-associated degradation. This gene is a strong tumor suppressor for colorectal carcinoma, and downregulation of it may serve as a good biomarker for predicting patient outcomes. Several transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DNAJB4-201 | ENST00000370763.6 | 2903 | 337aa | Protein coding | CCDS684 | Q9UDY4 | NM_007034.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DNAJB4-202 | ENST00000426517.1 | 686 | 172aa | Protein coding | - | C9JUL4 | - | CDS 3' incomplete, TSL:3, |
| DNAJB4-206 | ENST00000487931.1 | 949 | No protein | Processed transcript | - | - | - | TSL:2, |
| DNAJB4-205 | ENST00000484662.1 | 906 | No protein | Processed transcript | - | - | - | TSL:2, |
| DNAJB4-204 | ENST00000477671.2 | 737 | No protein | Processed transcript | - | - | - | TSL:5, |
| DNAJB4-203 | ENST00000476396.1 | 673 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DNAJB4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DNAJB4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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