CRISPR-U™ Gene Knockout Cell Line Strategy

DNAJB4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DNAJB4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DNAJB4
Gene id 11080
Organism Homo sapiens
Official full symbol DnaJ heat shock protein family (Hsp40) member B4
Gene type protein-coding
Also known as DNAJW, DjB4, HLJ1
Summary The protein encoded by this gene is a molecular chaperone, tumor suppressor, and member of the heat shock protein-40 family. The encoded protein binds the cell adhesion protein E-cadherin and targets it to the plasma membrane. This protein also binds incorrectly folded E-cadherin and targets it for endoplasmic reticulum-associated degradation. This gene is a strong tumor suppressor for colorectal carcinoma, and downregulation of it may serve as a good biomarker for predicting patient outcomes. Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DNAJB4-201 ENST00000370763.6 2903 337aa Protein coding CCDS684 Q9UDY4 NM_007034.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DNAJB4-202 ENST00000426517.1 686 172aa Protein coding - C9JUL4 - CDS 3' incomplete, TSL:3,
DNAJB4-206 ENST00000487931.1 949 No protein Processed transcript - - - TSL:2,
DNAJB4-205 ENST00000484662.1 906 No protein Processed transcript - - - TSL:2,
DNAJB4-204 ENST00000477671.2 737 No protein Processed transcript - - - TSL:5,
DNAJB4-203 ENST00000476396.1 673 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DNAJB4
This KO Strategy loading
Red Cotton™ Notes Gene DNAJB4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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