CRISPR-U™ Gene Knockout Cell Line Strategy

CHD1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CHD1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CHD1
Gene id 1105
Organism Homo sapiens
Official full symbol chromodomain helicase DNA binding protein 1
Gene type protein-coding
Also known as CHD-1, PILBOS
Summary The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.
Genomic regions Chromosome 5
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CHD1-212 ENST00000614616.5 8145 1710aa Protein coding CCDS34204 O14646-1 NM_001270.4 TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92,
CHD1-201 ENST00000284049.7 6457 1710aa Protein coding CCDS34204 O14646-1 - TSL:1, GENCODE basic, APPRIS P1,
CHD1-206 ENST00000511067.2 817 272aa Protein coding - A0A087WVF4 - CDS 5' and 3' incomplete, TSL:5,
CHD1-209 ENST00000512844.5 848 22aa Nonsense mediated decay - H0Y8Z0 - CDS 5' incomplete, TSL:5,
CHD1-203 ENST00000505657.1 736 113aa Nonsense mediated decay - H0Y8V4 - CDS 5' incomplete, TSL:3,
CHD1-211 ENST00000514344.5 2955 No protein Retained intron - - - TSL:5,
CHD1-208 ENST00000512392.5 1968 No protein Retained intron - - - TSL:2,
CHD1-210 ENST00000513064.1 786 No protein Retained intron - - - TSL:2,
CHD1-204 ENST00000505982.1 758 No protein Retained intron - - - TSL:3,
CHD1-205 ENST00000508756.2 724 No protein Retained intron - - - TSL:5,
CHD1-207 ENST00000511628.1 599 No protein Retained intron - - - TSL:3,
CHD1-202 ENST00000414220.2 434 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CHD1
This KO Strategy loading
Red Cotton™ Notes Gene CHD1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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