CRISPR-U™ Gene Knockout Cell Line
Strategy
CHD1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CHD1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CHD1 |
| Gene id | 1105 |
| Organism | Homo sapiens |
| Official full symbol | chromodomain helicase DNA binding protein 1 |
| Gene type | protein-coding |
| Also known as | CHD-1, PILBOS |
| Summary | The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CHD1-212 | ENST00000614616.5 | 8145 | 1710aa | Protein coding | CCDS34204 | O14646-1 | NM_001270.4 | TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CHD1-201 | ENST00000284049.7 | 6457 | 1710aa | Protein coding | CCDS34204 | O14646-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CHD1-206 | ENST00000511067.2 | 817 | 272aa | Protein coding | - | A0A087WVF4 | - | CDS 5' and 3' incomplete, TSL:5, |
| CHD1-209 | ENST00000512844.5 | 848 | 22aa | Nonsense mediated decay | - | H0Y8Z0 | - | CDS 5' incomplete, TSL:5, |
| CHD1-203 | ENST00000505657.1 | 736 | 113aa | Nonsense mediated decay | - | H0Y8V4 | - | CDS 5' incomplete, TSL:3, |
| CHD1-211 | ENST00000514344.5 | 2955 | No protein | Retained intron | - | - | - | TSL:5, |
| CHD1-208 | ENST00000512392.5 | 1968 | No protein | Retained intron | - | - | - | TSL:2, |
| CHD1-210 | ENST00000513064.1 | 786 | No protein | Retained intron | - | - | - | TSL:2, |
| CHD1-204 | ENST00000505982.1 | 758 | No protein | Retained intron | - | - | - | TSL:3, |
| CHD1-205 | ENST00000508756.2 | 724 | No protein | Retained intron | - | - | - | TSL:5, |
| CHD1-207 | ENST00000511628.1 | 599 | No protein | Retained intron | - | - | - | TSL:3, |
| CHD1-202 | ENST00000414220.2 | 434 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CHD1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CHD1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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