CRISPR-U™ Gene Knockout Cell Line
Strategy
LILRB4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LILRB4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LILRB4 |
| Gene id | 11006 |
| Organism | Homo sapiens |
| Official full symbol | leukocyte immunoglobulin like receptor B4 |
| Gene type | protein-coding |
| Also known as | CD85K, ILT-3, ILT3, LIR-5, LIR5 |
| Summary | This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LILRB4-204 | ENST00000391736.5 | 4002 | 448aa | Protein coding | CCDS12902 | Q8NHJ6 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| LILRB4-205 | ENST00000430952.6 | 1761 | 447aa | Protein coding | CCDS42618 | A0A0A0MSZ8 | - | TSL:1, GENCODE basic, APPRIS P4, |
| LILRB4-202 | ENST00000391733.7 | 1742 | 449aa | Protein coding | - | A0A0A0MS20 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| LILRB4-203 | ENST00000391734.7 | 1602 | 395aa | Protein coding | - | A8MUE1 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| LILRB4-206 | ENST00000434286.1 | 1204 | 399aa | Protein coding | - | C9JST2 | - | CDS 3' incomplete, TSL:5, |
| LILRB4-201 | ENST00000270452.6 | 539 | 131aa | Protein coding | - | A0A0A0MQW7 | - | CDS 3' incomplete, TSL:5, |
| LILRB4-210 | ENST00000494796.5 | 1272 | 63aa | Nonsense mediated decay | - | A0A087X0F8 | - | TSL:5, |
| LILRB4-208 | ENST00000461839.1 | 1645 | No protein | Processed transcript | - | - | - | TSL:1, |
| LILRB4-207 | ENST00000461262.1 | 430 | No protein | Processed transcript | - | - | - | TSL:3, |
| LILRB4-209 | ENST00000470943.1 | 945 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LILRB4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LILRB4 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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