CRISPR-U™ Gene Knockout Cell Line Strategy

LILRB4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LILRB4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LILRB4
Gene id 11006
Organism Homo sapiens
Official full symbol leukocyte immunoglobulin like receptor B4
Gene type protein-coding
Also known as CD85K, ILT-3, ILT3, LIR-5, LIR5
Summary This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LILRB4-204 ENST00000391736.5 4002 448aa Protein coding CCDS12902 Q8NHJ6 - TSL:5, GENCODE basic, APPRIS ALT2,
LILRB4-205 ENST00000430952.6 1761 447aa Protein coding CCDS42618 A0A0A0MSZ8 - TSL:1, GENCODE basic, APPRIS P4,
LILRB4-202 ENST00000391733.7 1742 449aa Protein coding - A0A0A0MS20 - TSL:5, GENCODE basic, APPRIS ALT2,
LILRB4-203 ENST00000391734.7 1602 395aa Protein coding - A8MUE1 - TSL:5, GENCODE basic, APPRIS ALT2,
LILRB4-206 ENST00000434286.1 1204 399aa Protein coding - C9JST2 - CDS 3' incomplete, TSL:5,
LILRB4-201 ENST00000270452.6 539 131aa Protein coding - A0A0A0MQW7 - CDS 3' incomplete, TSL:5,
LILRB4-210 ENST00000494796.5 1272 63aa Nonsense mediated decay - A0A087X0F8 - TSL:5,
LILRB4-208 ENST00000461839.1 1645 No protein Processed transcript - - - TSL:1,
LILRB4-207 ENST00000461262.1 430 No protein Processed transcript - - - TSL:3,
LILRB4-209 ENST00000470943.1 945 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LILRB4
This KO Strategy loading
Red Cotton™ Notes Gene LILRB4 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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