CRISPR-U™ Gene Knockout Cell Line Strategy

SLC27A4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SLC27A4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SLC27A4
Gene id 10999
Organism Homo sapiens
Official full symbol solute carrier family 27 member 4
Gene type protein-coding
Also known as ACSVL4, FATP4, IPS
Summary This gene encodes a member of a family of fatty acid transport proteins, which are involved in translocation of long-chain fatty acids cross the plasma membrane. This protein is expressed at high levels on the apical side of mature enterocytes in the small intestine, and appears to be the principal fatty acid transporter in enterocytes. Clinical studies suggest this gene as a candidate gene for the insulin resistance syndrome. Mutations in this gene have been associated with ichthyosis prematurity syndrome.
Genomic regions Chromosome 9
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SLC27A4-201 ENST00000300456.5 3229 643aa Protein coding CCDS6899 Q6P1M0-1 NM_005094.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SLC27A4-202 ENST00000372870.5 1557 237aa Protein coding - Q6P1M0-2 - TSL:1, GENCODE basic,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SLC27A4
This KO Strategy loading
Red Cotton™ Notes Gene SLC27A4 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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