CRISPR-U™ Gene Knockout Cell Line
Strategy
SLC27A4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SLC27A4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SLC27A4 |
| Gene id | 10999 |
| Organism | Homo sapiens |
| Official full symbol | solute carrier family 27 member 4 |
| Gene type | protein-coding |
| Also known as | ACSVL4, FATP4, IPS |
| Summary | This gene encodes a member of a family of fatty acid transport proteins, which are involved in translocation of long-chain fatty acids cross the plasma membrane. This protein is expressed at high levels on the apical side of mature enterocytes in the small intestine, and appears to be the principal fatty acid transporter in enterocytes. Clinical studies suggest this gene as a candidate gene for the insulin resistance syndrome. Mutations in this gene have been associated with ichthyosis prematurity syndrome. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SLC27A4-201 | ENST00000300456.5 | 3229 | 643aa | Protein coding | CCDS6899 | Q6P1M0-1 | NM_005094.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SLC27A4-202 | ENST00000372870.5 | 1557 | 237aa | Protein coding | - | Q6P1M0-2 | - | TSL:1, GENCODE basic, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SLC27A4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SLC27A4 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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