CRISPR-U™ Gene Knockout Cell Line Strategy

CCNI Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CCNI Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CCNI
Gene id 10983
Organism Homo sapiens
Official full symbol cyclin I
Gene type protein-coding
Also known as CCNI1, CYC1, CYI
Summary The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin shows the highest similarity with cyclin G. The transcript of this gene was found to be expressed constantly during cell cycle progression.
Genomic regions Chromosome 4
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CCNI-201 ENST00000237654.9 2759 377aa Protein coding CCDS3580 Q14094-1 NM_006835.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CCNI-205 ENST00000507788.2 915 49aa Protein coding - D6REZ3 - CDS 3' incomplete, TSL:4,
CCNI-208 ENST00000515468.1 636 177aa Protein coding - H0YA27 - CDS 5' incomplete, TSL:3,
CCNI-203 ENST00000505609.5 541 125aa Protein coding - D6RG12 - CDS 3' incomplete, TSL:4,
CCNI-207 ENST00000513774.1 517 80aa Protein coding - D6RH01 - CDS 3' incomplete, TSL:4,
CCNI-206 ENST00000511943.5 576 56aa Nonsense mediated decay - D6RDK3 - TSL:3,
CCNI-202 ENST00000504697.1 610 No protein Processed transcript - - - TSL:4,
CCNI-204 ENST00000506614.1 525 No protein Retained intron - - - TSL:2,
CCNI-209 ENST00000515790.1 390 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CCNI
This KO Strategy loading
Red Cotton™ Notes Gene CCNI had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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