CRISPR-U™ Gene Knockout Cell Line
Strategy
CCNI Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CCNI Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CCNI |
| Gene id | 10983 |
| Organism | Homo sapiens |
| Official full symbol | cyclin I |
| Gene type | protein-coding |
| Also known as | CCNI1, CYC1, CYI |
| Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin shows the highest similarity with cyclin G. The transcript of this gene was found to be expressed constantly during cell cycle progression. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CCNI-201 | ENST00000237654.9 | 2759 | 377aa | Protein coding | CCDS3580 | Q14094-1 | NM_006835.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CCNI-205 | ENST00000507788.2 | 915 | 49aa | Protein coding | - | D6REZ3 | - | CDS 3' incomplete, TSL:4, |
| CCNI-208 | ENST00000515468.1 | 636 | 177aa | Protein coding | - | H0YA27 | - | CDS 5' incomplete, TSL:3, |
| CCNI-203 | ENST00000505609.5 | 541 | 125aa | Protein coding | - | D6RG12 | - | CDS 3' incomplete, TSL:4, |
| CCNI-207 | ENST00000513774.1 | 517 | 80aa | Protein coding | - | D6RH01 | - | CDS 3' incomplete, TSL:4, |
| CCNI-206 | ENST00000511943.5 | 576 | 56aa | Nonsense mediated decay | - | D6RDK3 | - | TSL:3, |
| CCNI-202 | ENST00000504697.1 | 610 | No protein | Processed transcript | - | - | - | TSL:4, |
| CCNI-204 | ENST00000506614.1 | 525 | No protein | Retained intron | - | - | - | TSL:2, |
| CCNI-209 | ENST00000515790.1 | 390 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CCNI |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CCNI had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: