CRISPR-U™ Gene Knockout Cell Line
Strategy
IFI44L Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFI44L Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IFI44L |
| Gene id | 10964 |
| Organism | Homo sapiens |
| Official full symbol | interferon induced protein 44 like |
| Gene type | protein-coding |
| Also known as | C1orf29, GS3686 |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IFI44L-201 | ENST00000370751.10 | 5829 | 452aa | Protein coding | CCDS687 | Q53G44-1 | NM_006820.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IFI44L-212 | ENST00000679848.1 | 2007 | 194aa | Protein coding | - | B4E019 | - | GENCODE basic, |
| IFI44L-213 | ENST00000679998.1 | 1713 | 194aa | Protein coding | - | B4E019 | - | GENCODE basic, |
| IFI44L-204 | ENST00000459784.6 | 1682 | 194aa | Protein coding | - | B4E019 | - | TSL:3, GENCODE basic, |
| IFI44L-218 | ENST00000681613.1 | 1447 | 194aa | Protein coding | - | B4E019 | - | GENCODE basic, |
| IFI44L-214 | ENST00000680110.1 | 1315 | 194aa | Protein coding | - | B4E019 | - | GENCODE basic, |
| IFI44L-215 | ENST00000680295.1 | 1200 | 194aa | Protein coding | - | B4E019 | - | GENCODE basic, |
| IFI44L-202 | ENST00000450498.1 | 849 | 232aa | Protein coding | - | H7C1K6 | - | CDS 5' incomplete, TSL:5, |
| IFI44L-203 | ENST00000452835.1 | 554 | 97aa | Protein coding | - | C9JPJ0 | - | CDS 3' incomplete, TSL:4, |
| IFI44L-217 | ENST00000681501.1 | 1412 | 131aa | Nonsense mediated decay | - | - | - | - |
| IFI44L-207 | ENST00000476521.5 | 1162 | 47aa | Nonsense mediated decay | - | - | - | TSL:5, |
| IFI44L-205 | ENST00000462041.1 | 565 | No protein | Processed transcript | - | - | - | TSL:5, |
| IFI44L-209 | ENST00000486882.5 | 5152 | No protein | Retained intron | - | - | - | TSL:1, |
| IFI44L-210 | ENST00000494910.2 | 3140 | No protein | Retained intron | - | - | - | TSL:3, |
| IFI44L-208 | ENST00000476876.6 | 2520 | No protein | Retained intron | - | - | - | TSL:5, |
| IFI44L-211 | ENST00000679651.1 | 2035 | No protein | Retained intron | - | - | - | - |
| IFI44L-216 | ENST00000680621.1 | 1486 | No protein | Retained intron | - | - | - | - |
| IFI44L-206 | ENST00000474002.1 | 928 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IFI44L |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IFI44L had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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