CRISPR-U™ Gene Knockout Cell Line Strategy

IFI44L Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IFI44L Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IFI44L
Gene id 10964
Organism Homo sapiens
Official full symbol interferon induced protein 44 like
Gene type protein-coding
Also known as C1orf29, GS3686
Genomic regions Chromosome 1
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IFI44L-201 ENST00000370751.10 5829 452aa Protein coding CCDS687 Q53G44-1 NM_006820.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IFI44L-212 ENST00000679848.1 2007 194aa Protein coding - B4E019 - GENCODE basic,
IFI44L-213 ENST00000679998.1 1713 194aa Protein coding - B4E019 - GENCODE basic,
IFI44L-204 ENST00000459784.6 1682 194aa Protein coding - B4E019 - TSL:3, GENCODE basic,
IFI44L-218 ENST00000681613.1 1447 194aa Protein coding - B4E019 - GENCODE basic,
IFI44L-214 ENST00000680110.1 1315 194aa Protein coding - B4E019 - GENCODE basic,
IFI44L-215 ENST00000680295.1 1200 194aa Protein coding - B4E019 - GENCODE basic,
IFI44L-202 ENST00000450498.1 849 232aa Protein coding - H7C1K6 - CDS 5' incomplete, TSL:5,
IFI44L-203 ENST00000452835.1 554 97aa Protein coding - C9JPJ0 - CDS 3' incomplete, TSL:4,
IFI44L-217 ENST00000681501.1 1412 131aa Nonsense mediated decay - - - -
IFI44L-207 ENST00000476521.5 1162 47aa Nonsense mediated decay - - - TSL:5,
IFI44L-205 ENST00000462041.1 565 No protein Processed transcript - - - TSL:5,
IFI44L-209 ENST00000486882.5 5152 No protein Retained intron - - - TSL:1,
IFI44L-210 ENST00000494910.2 3140 No protein Retained intron - - - TSL:3,
IFI44L-208 ENST00000476876.6 2520 No protein Retained intron - - - TSL:5,
IFI44L-211 ENST00000679651.1 2035 No protein Retained intron - - - -
IFI44L-216 ENST00000680621.1 1486 No protein Retained intron - - - -
IFI44L-206 ENST00000474002.1 928 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IFI44L
This KO Strategy loading
Red Cotton™ Notes Gene IFI44L had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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