CRISPR-U™ Gene Knockout Cell Line Strategy

RALBP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RALBP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RALBP1
Gene id 10928
Organism Homo sapiens
Official full symbol ralA binding protein 1
Gene type protein-coding
Also known as RIP1, RLIP1, RLIP76
Summary RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 ).
Genomic regions Chromosome 18
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RALBP1-202 ENST00000383432.8 4379 655aa Protein coding CCDS11845 Q15311 NM_006788.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
RALBP1-201 ENST00000019317.8 4368 655aa Protein coding CCDS11845 Q15311 - TSL:1, GENCODE basic, APPRIS P1,
RALBP1-206 ENST00000609094.2 3676 242aa Protein coding - A0A1W2PRI7 - TSL:2, GENCODE basic,
RALBP1-205 ENST00000585015.6 945 186aa Protein coding - J3QLT3 - CDS 3' incomplete, TSL:3,
RALBP1-203 ENST00000458039.3 836 207aa Protein coding - E7ENF8 - CDS 3' incomplete, TSL:3,
RALBP1-204 ENST00000577221.1 503 3aa Protein coding - - - CDS 3' incomplete, TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RALBP1
This KO Strategy loading
Red Cotton™ Notes Gene RALBP1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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$49

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Single gRNA lentivirus

Work flow
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