CRISPR-U™ Gene Knockout Cell Line
Strategy
RALBP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RALBP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | RALBP1 |
| Gene id | 10928 |
| Organism | Homo sapiens |
| Official full symbol | ralA binding protein 1 |
| Gene type | protein-coding |
| Also known as | RIP1, RLIP1, RLIP76 |
| Summary | RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 ). |
| Genomic regions | Chromosome 18 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| RALBP1-202 | ENST00000383432.8 | 4379 | 655aa | Protein coding | CCDS11845 | Q15311 | NM_006788.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| RALBP1-201 | ENST00000019317.8 | 4368 | 655aa | Protein coding | CCDS11845 | Q15311 | - | TSL:1, GENCODE basic, APPRIS P1, |
| RALBP1-206 | ENST00000609094.2 | 3676 | 242aa | Protein coding | - | A0A1W2PRI7 | - | TSL:2, GENCODE basic, |
| RALBP1-205 | ENST00000585015.6 | 945 | 186aa | Protein coding | - | J3QLT3 | - | CDS 3' incomplete, TSL:3, |
| RALBP1-203 | ENST00000458039.3 | 836 | 207aa | Protein coding | - | E7ENF8 | - | CDS 3' incomplete, TSL:3, |
| RALBP1-204 | ENST00000577221.1 | 503 | 3aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | RALBP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene RALBP1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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