CRISPR-U™ Gene Knockout Cell Line
Strategy
FASTK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FASTK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FASTK |
| Gene id | 10922 |
| Organism | Homo sapiens |
| Official full symbol | Fas activated serine/threonine kinase |
| Gene type | protein-coding |
| Also known as | FAST |
| Summary | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FASTK-201 | ENST00000297532.11 | 1794 | 549aa | Protein coding | CCDS5918 | Q14296-1 | NM_006712.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| FASTK-212 | ENST00000482571.2 | 1764 | 522aa | Protein coding | CCDS59088 | Q14296-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| FASTK-202 | ENST00000353841.6 | 1402 | 408aa | Protein coding | CCDS5919 | Q14296-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| FASTK-217 | ENST00000540185.5 | 1609 | 465aa | Protein coding | - | G3V1R6 | - | TSL:5, GENCODE basic, |
| FASTK-215 | ENST00000489884.5 | 1751 | No protein | Processed transcript | - | - | - | TSL:1, |
| FASTK-210 | ENST00000478477.1 | 826 | No protein | Processed transcript | - | - | - | TSL:3, |
| FASTK-216 | ENST00000496663.1 | 818 | No protein | Processed transcript | - | - | - | TSL:2, |
| FASTK-205 | ENST00000461979.1 | 720 | No protein | Processed transcript | - | - | - | TSL:5, |
| FASTK-208 | ENST00000467237.5 | 3011 | No protein | Retained intron | - | - | - | TSL:1, |
| FASTK-209 | ENST00000469237.5 | 1904 | No protein | Retained intron | - | - | - | TSL:5, |
| FASTK-207 | ENST00000466855.1 | 1894 | No protein | Retained intron | - | - | - | TSL:5, |
| FASTK-213 | ENST00000482806.5 | 1826 | No protein | Retained intron | - | - | - | TSL:5, |
| FASTK-204 | ENST00000460980.5 | 1521 | No protein | Retained intron | - | - | - | TSL:5, |
| FASTK-214 | ENST00000483953.5 | 1178 | No protein | Retained intron | - | - | - | TSL:5, |
| FASTK-211 | ENST00000478883.1 | 706 | No protein | Retained intron | - | - | - | TSL:3, |
| FASTK-206 | ENST00000465272.1 | 457 | No protein | Retained intron | - | - | - | TSL:2, |
| FASTK-203 | ENST00000459800.1 | 449 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FASTK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FASTK had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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