CRISPR-U™ Gene Knockout Cell Line Strategy

FASTK Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FASTK Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FASTK
Gene id 10922
Organism Homo sapiens
Official full symbol Fas activated serine/threonine kinase
Gene type protein-coding
Also known as FAST
Summary The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined.
Genomic regions Chromosome 7
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FASTK-201 ENST00000297532.11 1794 549aa Protein coding CCDS5918 Q14296-1 NM_006712.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
FASTK-212 ENST00000482571.2 1764 522aa Protein coding CCDS59088 Q14296-3 - TSL:1, GENCODE basic, APPRIS ALT2,
FASTK-202 ENST00000353841.6 1402 408aa Protein coding CCDS5919 Q14296-2 - TSL:1, GENCODE basic, APPRIS ALT2,
FASTK-217 ENST00000540185.5 1609 465aa Protein coding - G3V1R6 - TSL:5, GENCODE basic,
FASTK-215 ENST00000489884.5 1751 No protein Processed transcript - - - TSL:1,
FASTK-210 ENST00000478477.1 826 No protein Processed transcript - - - TSL:3,
FASTK-216 ENST00000496663.1 818 No protein Processed transcript - - - TSL:2,
FASTK-205 ENST00000461979.1 720 No protein Processed transcript - - - TSL:5,
FASTK-208 ENST00000467237.5 3011 No protein Retained intron - - - TSL:1,
FASTK-209 ENST00000469237.5 1904 No protein Retained intron - - - TSL:5,
FASTK-207 ENST00000466855.1 1894 No protein Retained intron - - - TSL:5,
FASTK-213 ENST00000482806.5 1826 No protein Retained intron - - - TSL:5,
FASTK-204 ENST00000460980.5 1521 No protein Retained intron - - - TSL:5,
FASTK-214 ENST00000483953.5 1178 No protein Retained intron - - - TSL:5,
FASTK-211 ENST00000478883.1 706 No protein Retained intron - - - TSL:3,
FASTK-206 ENST00000465272.1 457 No protein Retained intron - - - TSL:2,
FASTK-203 ENST00000459800.1 449 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FASTK
This KO Strategy loading
Red Cotton™ Notes Gene FASTK had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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