CRISPR-U™ Gene Knockout Cell Line Strategy

MALT1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MALT1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MALT1
Gene id 10892
Organism Homo sapiens
Official full symbol MALT1 paracaspase
Gene type protein-coding
Also known as IMD12, MLT, MLT1, PCASP1
Summary This gene encodes a caspase-like protease that plays a role in BCL10-induced activation of NF-kappaB. The protein is a component of the CARMA1-BCL10-MALT1 (CBM) signalosome that triggers NF-kappaB signaling and lymphoctye activation following antigen-receptor stimulation. Mutations in this gene result in immunodeficiency 12 (IMD12). This gene has been found to be recurrently rearranged in chromosomal translocations with other genes in mucosa-associated lymphoid tissue lymphomas, including a t(11;18)(q21;q21) translocation with the baculoviral IAP repeat-containing protein 3 (also known as apoptosis inhibitor 2) locus , and a t(14;18)(q32;q21) translocation with the immunoglobulin heavy chain locus (IGH-MALT1). Alternatively spliced transcript variants have been described for this gene.
Genomic regions Chromosome 18
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MALT1-208 ENST00000649217.2 9289 824aa Protein coding CCDS11967 Q9UDY8-1 NM_006785.4 GENCODE basic, APPRIS P3, MANE Select v0.92,
MALT1-201 ENST00000345724.7 2866 813aa Protein coding CCDS11968 Q9UDY8-2 - TSL:1, GENCODE basic, APPRIS ALT2,
MALT1-204 ENST00000591792.1 565 119aa Protein coding - K7EP42 - CDS 3' incomplete, TSL:4,
MALT1-210 ENST00000650045.1 3312 468aa Nonsense mediated decay - A0A3B3IS32 - -
MALT1-205 ENST00000648670.1 2185 163aa Nonsense mediated decay - A0A3B3IS82 - CDS 5' incomplete,
MALT1-209 ENST00000649756.1 500 No protein Processed transcript - - - -
MALT1-207 ENST00000649202.1 2374 No protein Retained intron - - - -
MALT1-206 ENST00000649125.1 2277 No protein Retained intron - - - -
MALT1-203 ENST00000589873.5 2246 No protein Retained intron - - - TSL:2,
MALT1-211 ENST00000650355.1 1519 No protein Retained intron - - - -
MALT1-202 ENST00000587438.1 869 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MALT1
This KO Strategy loading
Red Cotton™ Notes Gene MALT1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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