CRISPR-U™ Gene Knockout Cell Line Strategy

CFHR3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CFHR3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CFHR3
Gene id 10878
Organism Homo sapiens
Official full symbol complement factor H related 3
Gene type protein-coding
Also known as CFHL3, DOWN16, FHR-3, FHR3, HLF4
Summary The protein encoded by this gene is a secreted protein, which belongs to the complement factor H-related protein family. It binds to heparin, and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-related macular degeneration, and with an increased risk of atypical hemolytic-uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CFHR3-201 ENST00000367425.9 2934 330aa Protein coding CCDS30958 Q02985-1 NM_021023.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CFHR3-203 ENST00000391985.7 1043 269aa Protein coding CCDS53453 Q02985-2 - TSL:2, GENCODE basic,
CFHR3-205 ENST00000471440.6 1965 232aa Protein coding - Q6NSD3 - TSL:1, GENCODE basic,
CFHR3-206 ENST00000617219.1 1462 195aa Protein coding - A0A087WYK9 - TSL:5, GENCODE basic,
CFHR3-202 ENST00000367427.7 1242 232aa Nonsense mediated decay - Q6NSD3 - TSL:5,
CFHR3-204 ENST00000461558.2 785 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CFHR3
This KO Strategy loading
Red Cotton™ Notes Gene CFHR3 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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