CRISPR-U™ Gene Knockout Cell Line
Strategy
PPP1R13L Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPP1R13L Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPP1R13L |
| Gene id | 10848 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase 1 regulatory subunit 13 like |
| Gene type | protein-coding |
| Also known as | IASPP, NKIP1, RAI, RAI4 |
| Summary | IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPP1R13L-201 | ENST00000360957.10 | 3131 | 828aa | Protein coding | CCDS33050 | A0A024R0Q5 Q8WUF5 | NM_006663.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPP1R13L-202 | ENST00000418234.6 | 3118 | 828aa | Protein coding | CCDS33050 | A0A024R0Q5 Q8WUF5 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PPP1R13L-207 | ENST00000591986.1 | 648 | 29aa | Protein coding | - | K7ENG6 | - | CDS 5' incomplete, TSL:3, |
| PPP1R13L-209 | ENST00000593226.5 | 525 | 128aa | Protein coding | - | K7EN03 | - | CDS 3' incomplete, TSL:3, |
| PPP1R13L-208 | ENST00000592134.1 | 392 | 60aa | Protein coding | - | K7EPP1 | - | CDS 3' incomplete, TSL:3, |
| PPP1R13L-205 | ENST00000589371.1 | 355 | 90aa | Protein coding | - | K7EML6 | - | CDS 5' incomplete, TSL:2, |
| PPP1R13L-204 | ENST00000587270.5 | 2512 | No protein | Retained intron | - | - | - | TSL:1, |
| PPP1R13L-203 | ENST00000585905.1 | 2176 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP1R13L-206 | ENST00000589858.1 | 610 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPP1R13L |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPP1R13L had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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