CRISPR-U™ Gene Knockout Cell Line Strategy

PPP1R13L Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PPP1R13L Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPP1R13L
Gene id 10848
Organism Homo sapiens
Official full symbol protein phosphatase 1 regulatory subunit 13 like
Gene type protein-coding
Also known as IASPP, NKIP1, RAI, RAI4
Summary IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53.
Genomic regions Chromosome 19
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPP1R13L-201 ENST00000360957.10 3131 828aa Protein coding CCDS33050 A0A024R0Q5 Q8WUF5 NM_006663.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PPP1R13L-202 ENST00000418234.6 3118 828aa Protein coding CCDS33050 A0A024R0Q5 Q8WUF5 - TSL:1, GENCODE basic, APPRIS P1,
PPP1R13L-207 ENST00000591986.1 648 29aa Protein coding - K7ENG6 - CDS 5' incomplete, TSL:3,
PPP1R13L-209 ENST00000593226.5 525 128aa Protein coding - K7EN03 - CDS 3' incomplete, TSL:3,
PPP1R13L-208 ENST00000592134.1 392 60aa Protein coding - K7EPP1 - CDS 3' incomplete, TSL:3,
PPP1R13L-205 ENST00000589371.1 355 90aa Protein coding - K7EML6 - CDS 5' incomplete, TSL:2,
PPP1R13L-204 ENST00000587270.5 2512 No protein Retained intron - - - TSL:1,
PPP1R13L-203 ENST00000585905.1 2176 No protein Retained intron - - - TSL:2,
PPP1R13L-206 ENST00000589858.1 610 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPP1R13L
This KO Strategy loading
Red Cotton™ Notes Gene PPP1R13L had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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