CRISPR-U™ Gene Knockout Cell Line
Strategy
CLPX Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CLPX Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CLPX |
| Gene id | 10845 |
| Organism | Homo sapiens |
| Official full symbol | caseinolytic mitochondrial matrix peptidase chaperone subunit X |
| Gene type | protein-coding |
| Also known as | EPP2 |
| Summary | The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CLPX-201 | ENST00000300107.7 | 4695 | 633aa | Protein coding | CCDS10202 | A0A024R5X7 O76031 | NM_006660.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CLPX-205 | ENST00000559218.1 | 533 | 130aa | Protein coding | - | H0YK07 | - | CDS 3' incomplete, TSL:4, |
| CLPX-204 | ENST00000559152.5 | 2228 | 268aa | Nonsense mediated decay | - | H0YM48 | - | TSL:1, |
| CLPX-203 | ENST00000558958.1 | 1493 | No protein | Retained intron | - | - | - | TSL:1, |
| CLPX-202 | ENST00000558103.1 | 605 | No protein | Retained intron | - | - | - | TSL:5, |
| CLPX-206 | ENST00000560166.1 | 563 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CLPX |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CLPX had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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