CRISPR-U™ Gene Knockout Cell Line Strategy

CLPX Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CLPX Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CLPX
Gene id 10845
Organism Homo sapiens
Official full symbol caseinolytic mitochondrial matrix peptidase chaperone subunit X
Gene type protein-coding
Also known as EPP2
Summary The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.
Genomic regions Chromosome 15
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CLPX-201 ENST00000300107.7 4695 633aa Protein coding CCDS10202 A0A024R5X7 O76031 NM_006660.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CLPX-205 ENST00000559218.1 533 130aa Protein coding - H0YK07 - CDS 3' incomplete, TSL:4,
CLPX-204 ENST00000559152.5 2228 268aa Nonsense mediated decay - H0YM48 - TSL:1,
CLPX-203 ENST00000558958.1 1493 No protein Retained intron - - - TSL:1,
CLPX-202 ENST00000558103.1 605 No protein Retained intron - - - TSL:5,
CLPX-206 ENST00000560166.1 563 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CLPX
This KO Strategy loading
Red Cotton™ Notes Gene CLPX had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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