CRISPR-U™ Gene Knockout Cell Line
Strategy
CEACAM3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CEACAM3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CEACAM3 |
| Gene id | 1084 |
| Organism | Homo sapiens |
| Official full symbol | CEA cell adhesion molecule 3 |
| Gene type | protein-coding |
| Also known as | CD66D, CEA, CGM1, W264, W282 |
| Summary | This gene encodes a member of the family of carcinoembryonic antigen-related cell adhesion molecules (CEACAMs), which are used by several bacterial pathogens to bind and invade host cells. The encoded transmembrane protein directs phagocytosis of several bacterial species that is dependent on the small GTPase Rac. It is thought to serve an important role in controlling human-specific pathogens by the innate immune system. Alternatively spliced transcript variants have been described. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CEACAM3-202 | ENST00000357396.8 | 1167 | 252aa | Protein coding | CCDS12586 | P40198-1 | NM_001815.5 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| CEACAM3-208 | ENST00000630848.2 | 1110 | 212aa | Protein coding | CCDS62685 | P40198-3 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| CEACAM3-201 | ENST00000344550.4 | 1022 | 212aa | Protein coding | CCDS62685 | P40198-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| CEACAM3-205 | ENST00000596544.1 | 474 | 99aa | Protein coding | - | M0QXR5 | - | CDS 3' incomplete, TSL:3, |
| CEACAM3-203 | ENST00000415495.5 | 1640 | 177aa | Nonsense mediated decay | - | P40198-2 | - | TSL:1, |
| CEACAM3-204 | ENST00000595255.1 | 1455 | No protein | Processed transcript | - | - | - | TSL:5, |
| CEACAM3-207 | ENST00000599662.1 | 850 | No protein | Processed transcript | - | - | - | TSL:5, |
| CEACAM3-206 | ENST00000599305.1 | 588 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CEACAM3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CEACAM3 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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