CRISPR-U™ Gene Knockout Cell Line Strategy

CEACAM3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CEACAM3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CEACAM3
Gene id 1084
Organism Homo sapiens
Official full symbol CEA cell adhesion molecule 3
Gene type protein-coding
Also known as CD66D, CEA, CGM1, W264, W282
Summary This gene encodes a member of the family of carcinoembryonic antigen-related cell adhesion molecules (CEACAMs), which are used by several bacterial pathogens to bind and invade host cells. The encoded transmembrane protein directs phagocytosis of several bacterial species that is dependent on the small GTPase Rac. It is thought to serve an important role in controlling human-specific pathogens by the innate immune system. Alternatively spliced transcript variants have been described.
Genomic regions Chromosome 19
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CEACAM3-202 ENST00000357396.8 1167 252aa Protein coding CCDS12586 P40198-1 NM_001815.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
CEACAM3-208 ENST00000630848.2 1110 212aa Protein coding CCDS62685 P40198-3 - TSL:5, GENCODE basic, APPRIS ALT2,
CEACAM3-201 ENST00000344550.4 1022 212aa Protein coding CCDS62685 P40198-3 - TSL:1, GENCODE basic, APPRIS ALT2,
CEACAM3-205 ENST00000596544.1 474 99aa Protein coding - M0QXR5 - CDS 3' incomplete, TSL:3,
CEACAM3-203 ENST00000415495.5 1640 177aa Nonsense mediated decay - P40198-2 - TSL:1,
CEACAM3-204 ENST00000595255.1 1455 No protein Processed transcript - - - TSL:5,
CEACAM3-207 ENST00000599662.1 850 No protein Processed transcript - - - TSL:5,
CEACAM3-206 ENST00000599305.1 588 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CEACAM3
This KO Strategy loading
Red Cotton™ Notes Gene CEACAM3 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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