CRISPR-U™ Gene Knockout Cell Line
Strategy
MTHFD2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MTHFD2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MTHFD2 |
| Gene id | 10797 |
| Organism | Homo sapiens |
| Official full symbol | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase |
| Gene type | protein-coding |
| Also known as | NMDMC |
| Summary | This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 13 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MTHFD2-201 | ENST00000394053.7 | 4403 | 350aa | Protein coding | CCDS1935 | P13995-1 | NM_006636.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MTHFD2-209 | ENST00000677170.1 | 3362 | 248aa | Protein coding | - | - | - | GENCODE basic, |
| MTHFD2-213 | ENST00000677997.1 | 3340 | 324aa | Protein coding | - | - | - | GENCODE basic, |
| MTHFD2-218 | ENST00000678684.1 | 2710 | 248aa | Protein coding | - | - | - | GENCODE basic, |
| MTHFD2-219 | ENST00000678731.1 | 2309 | 248aa | Protein coding | - | - | - | GENCODE basic, |
| MTHFD2-221 | ENST00000679055.1 | 2276 | 248aa | Protein coding | - | - | - | GENCODE basic, |
| MTHFD2-215 | ENST00000678340.1 | 2057 | 212aa | Protein coding | - | - | - | GENCODE basic, |
| MTHFD2-202 | ENST00000409601.1 | 1865 | 267aa | Protein coding | - | B8ZZU9 | - | TSL:5, GENCODE basic, |
| MTHFD2-216 | ENST00000678623.1 | 1834 | 248aa | Protein coding | - | - | - | GENCODE basic, |
| MTHFD2-203 | ENST00000409804.5 | 1757 | 222aa | Protein coding | - | B9A062 | - | TSL:5, GENCODE basic, |
| MTHFD2-212 | ENST00000677558.1 | 1243 | 36aa | Protein coding | - | - | - | GENCODE basic, |
| MTHFD2-217 | ENST00000678633.1 | 1239 | 36aa | Protein coding | - | - | - | GENCODE basic, |
| MTHFD2-204 | ENST00000462026.2 | 895 | 203aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:5, |
| MTHFD2-211 | ENST00000677377.1 | 4033 | 36aa | Nonsense mediated decay | - | - | - | - |
| MTHFD2-208 | ENST00000489041.2 | 2463 | 38aa | Nonsense mediated decay | - | F8WF06 | - | TSL:2, |
| MTHFD2-214 | ENST00000678228.1 | 2320 | 38aa | Nonsense mediated decay | - | - | - | - |
| MTHFD2-205 | ENST00000470592.5 | 1396 | 37aa | Nonsense mediated decay | - | F8WF89 | - | TSL:2, |
| MTHFD2-206 | ENST00000477455.5 | 696 | No protein | Processed transcript | - | - | - | TSL:3, |
| MTHFD2-220 | ENST00000678955.1 | 6506 | No protein | Retained intron | - | - | - | - |
| MTHFD2-210 | ENST00000677299.1 | 2438 | No protein | Retained intron | - | - | - | - |
| MTHFD2-207 | ENST00000488086.1 | 483 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MTHFD2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MTHFD2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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