CRISPR-U™ Gene Knockout Cell Line Strategy

MTHFD2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MTHFD2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MTHFD2
Gene id 10797
Organism Homo sapiens
Official full symbol methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Gene type protein-coding
Also known as NMDMC
Summary This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
Genomic regions Chromosome 2
Strategy Summary
This gene has 13 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MTHFD2-201 ENST00000394053.7 4403 350aa Protein coding CCDS1935 P13995-1 NM_006636.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MTHFD2-209 ENST00000677170.1 3362 248aa Protein coding - - - GENCODE basic,
MTHFD2-213 ENST00000677997.1 3340 324aa Protein coding - - - GENCODE basic,
MTHFD2-218 ENST00000678684.1 2710 248aa Protein coding - - - GENCODE basic,
MTHFD2-219 ENST00000678731.1 2309 248aa Protein coding - - - GENCODE basic,
MTHFD2-221 ENST00000679055.1 2276 248aa Protein coding - - - GENCODE basic,
MTHFD2-215 ENST00000678340.1 2057 212aa Protein coding - - - GENCODE basic,
MTHFD2-202 ENST00000409601.1 1865 267aa Protein coding - B8ZZU9 - TSL:5, GENCODE basic,
MTHFD2-216 ENST00000678623.1 1834 248aa Protein coding - - - GENCODE basic,
MTHFD2-203 ENST00000409804.5 1757 222aa Protein coding - B9A062 - TSL:5, GENCODE basic,
MTHFD2-212 ENST00000677558.1 1243 36aa Protein coding - - - GENCODE basic,
MTHFD2-217 ENST00000678633.1 1239 36aa Protein coding - - - GENCODE basic,
MTHFD2-204 ENST00000462026.2 895 203aa Protein coding - - - CDS 3' incomplete, TSL:5,
MTHFD2-211 ENST00000677377.1 4033 36aa Nonsense mediated decay - - - -
MTHFD2-208 ENST00000489041.2 2463 38aa Nonsense mediated decay - F8WF06 - TSL:2,
MTHFD2-214 ENST00000678228.1 2320 38aa Nonsense mediated decay - - - -
MTHFD2-205 ENST00000470592.5 1396 37aa Nonsense mediated decay - F8WF89 - TSL:2,
MTHFD2-206 ENST00000477455.5 696 No protein Processed transcript - - - TSL:3,
MTHFD2-220 ENST00000678955.1 6506 No protein Retained intron - - - -
MTHFD2-210 ENST00000677299.1 2438 No protein Retained intron - - - -
MTHFD2-207 ENST00000488086.1 483 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MTHFD2
This KO Strategy loading
Red Cotton™ Notes Gene MTHFD2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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