CRISPR-U™ Gene Knockout Cell Line Strategy

WDR4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human WDR4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol WDR4
Gene id 10785
Organism Homo sapiens
Official full symbol WD repeat domain 4
Gene type protein-coding
Also known as GAMOS6, MIGSB, TRM82, TRMT82, hWH
Summary This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Alternatively spliced transcript variants have been found for this gene.
Genomic regions Chromosome 21
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
WDR4-202 ENST00000398208.3 2106 412aa Protein coding CCDS13691 P57081-1 NM_018669.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
WDR4-201 ENST00000330317.6 1471 412aa Protein coding CCDS13691 P57081-1 - TSL:1, GENCODE basic, APPRIS P1,
WDR4-207 ENST00000492742.5 2205 No protein Processed transcript - - - TSL:2,
WDR4-205 ENST00000476326.5 1977 No protein Processed transcript - - - TSL:1,
WDR4-204 ENST00000470658.1 901 No protein Processed transcript - - - TSL:5,
WDR4-206 ENST00000479429.5 759 No protein Processed transcript - - - TSL:5,
WDR4-203 ENST00000463902.5 609 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene WDR4
This KO Strategy loading
Red Cotton™ Notes Gene WDR4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×