CRISPR-U™ Gene Knockout Cell Line
Strategy
WDR4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human WDR4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | WDR4 |
| Gene id | 10785 |
| Organism | Homo sapiens |
| Official full symbol | WD repeat domain 4 |
| Gene type | protein-coding |
| Also known as | GAMOS6, MIGSB, TRM82, TRMT82, hWH |
| Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Alternatively spliced transcript variants have been found for this gene. |
| Genomic regions | Chromosome 21 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| WDR4-202 | ENST00000398208.3 | 2106 | 412aa | Protein coding | CCDS13691 | P57081-1 | NM_018669.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| WDR4-201 | ENST00000330317.6 | 1471 | 412aa | Protein coding | CCDS13691 | P57081-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| WDR4-207 | ENST00000492742.5 | 2205 | No protein | Processed transcript | - | - | - | TSL:2, |
| WDR4-205 | ENST00000476326.5 | 1977 | No protein | Processed transcript | - | - | - | TSL:1, |
| WDR4-204 | ENST00000470658.1 | 901 | No protein | Processed transcript | - | - | - | TSL:5, |
| WDR4-206 | ENST00000479429.5 | 759 | No protein | Processed transcript | - | - | - | TSL:5, |
| WDR4-203 | ENST00000463902.5 | 609 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | WDR4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene WDR4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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