CRISPR-U™ Gene Knockout Cell Line Strategy

ARPP19 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ARPP19 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ARPP19
Gene id 10776
Organism Homo sapiens
Official full symbol cAMP regulated phosphoprotein 19
Gene type protein-coding
Also known as ARPP-16, ARPP-19, ARPP16, ENSAL
Summary The 19-kD cAMP-regulated phosphoprotein plays a role in regulating mitosis by inhibiting protein phosphatase-2A (PP2A; see MIM 176915) (summary by Gharbi-Ayachi et al., 2010 ).
Genomic regions Chromosome 15
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ARPP19-201 ENST00000249822.9 5366 112aa Protein coding CCDS32242 P56211-1 NM_006628.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ARPP19-208 ENST00000566423.5 5362 112aa Protein coding CCDS32242 P56211-1 - TSL:1, GENCODE basic, APPRIS P1,
ARPP19-202 ENST00000561650.5 3245 96aa Protein coding CCDS81883 P56211-2 - TSL:2, GENCODE basic,
ARPP19-211 ENST00000568196.1 1489 96aa Protein coding CCDS81883 P56211-2 - TSL:2, GENCODE basic,
ARPP19-204 ENST00000563277.5 955 96aa Protein coding CCDS81883 P56211-2 - TSL:2, GENCODE basic,
ARPP19-203 ENST00000561971.1 799 131aa Protein coding CCDS76757 H3BMD8 - TSL:3, GENCODE basic,
ARPP19-209 ENST00000567669.5 745 112aa Protein coding CCDS32242 P56211-1 - TSL:2, GENCODE basic, APPRIS P1,
ARPP19-206 ENST00000564163.5 718 131aa Protein coding CCDS76757 H3BMD8 - TSL:2, GENCODE basic,
ARPP19-212 ENST00000569281.6 661 112aa Protein coding CCDS32242 P56211-1 - TSL:4, GENCODE basic, APPRIS P1,
ARPP19-205 ENST00000563566.5 568 96aa Protein coding CCDS81883 P56211-2 - TSL:4, GENCODE basic,
ARPP19-213 ENST00000569723.5 550 71aa Protein coding - H3BQ52 - TSL:4, GENCODE basic,
ARPP19-210 ENST00000567830.1 275 72aa Protein coding - H3BTD3 - CDS 3' incomplete, TSL:3,
ARPP19-207 ENST00000565288.1 242 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ARPP19
This KO Strategy loading
Red Cotton™ Notes Gene ARPP19 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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