CRISPR-U™ Gene Knockout Cell Line
Strategy
PLK2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PLK2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PLK2 |
| Gene id | 10769 |
| Organism | Homo sapiens |
| Official full symbol | polo like kinase 2 |
| Gene type | protein-coding |
| Also known as | SNK, hPlk2, hSNK |
| Summary | The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PLK2-215 | ENST00000617412.1 | 2792 | 671aa | Protein coding | CCDS75250 | A0A087WUH9 | - | TSL:5, GENCODE basic, |
| PLK2-201 | ENST00000274289.8 | 2786 | 685aa | Protein coding | CCDS3974 | A0A024QZV1 Q9NYY3 | NM_006622.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PLK2-202 | ENST00000502671.5 | 768 | No protein | Processed transcript | - | - | - | TSL:5, |
| PLK2-214 | ENST00000515415.5 | 1410 | No protein | Retained intron | - | - | - | TSL:5, |
| PLK2-213 | ENST00000514306.1 | 823 | No protein | Retained intron | - | - | - | TSL:2, |
| PLK2-205 | ENST00000503713.1 | 690 | No protein | Retained intron | - | - | - | TSL:2, |
| PLK2-206 | ENST00000504196.1 | 625 | No protein | Retained intron | - | - | - | TSL:2, |
| PLK2-211 | ENST00000510629.1 | 591 | No protein | Retained intron | - | - | - | TSL:2, |
| PLK2-204 | ENST00000503378.1 | 587 | No protein | Retained intron | - | - | - | TSL:2, |
| PLK2-209 | ENST00000509422.1 | 580 | No protein | Retained intron | - | - | - | TSL:4, |
| PLK2-208 | ENST00000508300.1 | 558 | No protein | Retained intron | - | - | - | TSL:3, |
| PLK2-207 | ENST00000505244.1 | 557 | No protein | Retained intron | - | - | - | TSL:2, |
| PLK2-210 | ENST00000509555.5 | 556 | No protein | Retained intron | - | - | - | TSL:2, |
| PLK2-203 | ENST00000503115.5 | 536 | No protein | Retained intron | - | - | - | TSL:2, |
| PLK2-212 | ENST00000511326.1 | 275 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PLK2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PLK2 had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
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$599
Single gRNA lentivirus
Work flow
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