CRISPR-U™ Gene Knockout Cell Line Strategy

PLK2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PLK2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PLK2
Gene id 10769
Organism Homo sapiens
Official full symbol polo like kinase 2
Gene type protein-coding
Also known as SNK, hPlk2, hSNK
Summary The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 5
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PLK2-215 ENST00000617412.1 2792 671aa Protein coding CCDS75250 A0A087WUH9 - TSL:5, GENCODE basic,
PLK2-201 ENST00000274289.8 2786 685aa Protein coding CCDS3974 A0A024QZV1 Q9NYY3 NM_006622.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PLK2-202 ENST00000502671.5 768 No protein Processed transcript - - - TSL:5,
PLK2-214 ENST00000515415.5 1410 No protein Retained intron - - - TSL:5,
PLK2-213 ENST00000514306.1 823 No protein Retained intron - - - TSL:2,
PLK2-205 ENST00000503713.1 690 No protein Retained intron - - - TSL:2,
PLK2-206 ENST00000504196.1 625 No protein Retained intron - - - TSL:2,
PLK2-211 ENST00000510629.1 591 No protein Retained intron - - - TSL:2,
PLK2-204 ENST00000503378.1 587 No protein Retained intron - - - TSL:2,
PLK2-209 ENST00000509422.1 580 No protein Retained intron - - - TSL:4,
PLK2-208 ENST00000508300.1 558 No protein Retained intron - - - TSL:3,
PLK2-207 ENST00000505244.1 557 No protein Retained intron - - - TSL:2,
PLK2-210 ENST00000509555.5 556 No protein Retained intron - - - TSL:2,
PLK2-203 ENST00000503115.5 536 No protein Retained intron - - - TSL:2,
PLK2-212 ENST00000511326.1 275 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PLK2
This KO Strategy loading
Red Cotton™ Notes Gene PLK2 had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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