CRISPR-U™ Gene Knockout Cell Line Strategy

CTSC Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CTSC Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CTSC
Gene id 1075
Organism Homo sapiens
Official full symbol cathepsin C
Gene type protein-coding
Also known as CPPI, DPP-I, DPP1, DPPI, HMS, JP, JPD, PALS, PDON1, PLS
Summary This gene encodes a member of the peptidase C1 family and lysosomal cysteine proteinase that appears to be a central coordinator for activation of many serine proteinases in cells of the immune system. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate heavy and light chains that form a disulfide-linked dimer. A portion of the propeptide acts as an intramolecular chaperone for the folding and stabilization of the mature enzyme. This enzyme requires chloride ions for activity and can degrade glucagon. Defects in the encoded protein have been shown to be a cause of Papillon-Lefevre syndrome, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis.
Genomic regions Chromosome 11
Strategy Summary
This gene has 13 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CTSC-203 ENST00000524463.6 6144 137aa Protein coding CCDS31654 P53634-2 - TSL:1, GENCODE basic,
CTSC-201 ENST00000227266.10 1870 463aa Protein coding CCDS8282 P53634-1 NM_001814.6 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
CTSC-212 ENST00000677106.1 840 137aa Protein coding CCDS31654 - - GENCODE basic,
CTSC-206 ENST00000529974.2 778 141aa Protein coding CCDS44693 P53634-3 - TSL:1, GENCODE basic,
CTSC-230 ENST00000679224.1 2774 342aa Protein coding - - - GENCODE basic,
CTSC-223 ENST00000678506.1 1867 450aa Protein coding - - - GENCODE basic, APPRIS ALT2,
CTSC-222 ENST00000678464.1 1667 452aa Protein coding - - - GENCODE basic,
CTSC-229 ENST00000679199.1 1604 134aa Protein coding - - - GENCODE basic,
CTSC-204 ENST00000527018.6 1524 325aa Protein coding - H0YCY8 - TSL:5, GENCODE basic,
CTSC-227 ENST00000678915.1 1431 419aa Protein coding - - - GENCODE basic,
CTSC-202 ENST00000393301.5 900 165aa Protein coding - - - TSL:2, GENCODE basic,
CTSC-219 ENST00000677976.1 724 117aa Protein coding - - - GENCODE basic,
CTSC-205 ENST00000528020.2 669 21aa Protein coding - H0YDA2 - TSL:2, GENCODE basic,
CTSC-228 ENST00000679032.1 6215 150aa Nonsense mediated decay - - - -
CTSC-215 ENST00000677661.1 2468 135aa Nonsense mediated decay - - - -
CTSC-217 ENST00000677802.1 1957 135aa Nonsense mediated decay - - - -
CTSC-211 ENST00000676612.1 1828 48aa Nonsense mediated decay - - - -
CTSC-221 ENST00000678395.1 1802 143aa Nonsense mediated decay - - - -
CTSC-213 ENST00000677208.1 1662 108aa Nonsense mediated decay - - - -
CTSC-224 ENST00000678520.1 1607 48aa Nonsense mediated decay - - - -
CTSC-218 ENST00000677877.1 1239 75aa Nonsense mediated decay - - - -
CTSC-225 ENST00000678554.1 1206 302aa Nonsense mediated decay - - - -
CTSC-216 ENST00000677796.1 764 48aa Nonsense mediated decay - - - -
CTSC-214 ENST00000677468.1 677 60aa Nonsense mediated decay - - - -
CTSC-231 ENST00000679297.1 1087 No protein Processed transcript - - - -
CTSC-209 ENST00000534131.2 672 No protein Processed transcript - - - TSL:2,
CTSC-226 ENST00000678664.1 619 No protein Processed transcript - - - -
CTSC-207 ENST00000533865.6 7564 No protein Retained intron - - - TSL:4,
CTSC-208 ENST00000533897.2 6119 No protein Retained intron - - - TSL:2,
CTSC-210 ENST00000676529.1 1886 No protein Retained intron - - - -
CTSC-220 ENST00000678065.1 1587 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CTSC
This KO Strategy loading
Red Cotton™ Notes Gene CTSC had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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