CRISPR-U™ Gene Knockout Cell Line
Strategy
MAP3K2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAP3K2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAP3K2 |
| Gene id | 10746 |
| Organism | Homo sapiens |
| Official full symbol | mitogen-activated protein kinase kinase kinase 2 |
| Gene type | protein-coding |
| Also known as | MEKK2, MEKK2B |
| Summary | The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAP3K2-203 | ENST00000409947.5 | 10992 | 619aa | Protein coding | CCDS46404 | A0A024RAH0 Q9Y2U5 | - | TSL:1, GENCODE basic, APPRIS P1, |
| MAP3K2-201 | ENST00000344908.9 | 2135 | 619aa | Protein coding | CCDS46404 | A0A024RAH0 Q9Y2U5 | - | TSL:1, GENCODE basic, APPRIS P1, |
| MAP3K2-202 | ENST00000409179.2 | 689 | 47aa | Protein coding | - | F8W6K3 | - | CDS 3' incomplete, TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAP3K2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAP3K2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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