CRISPR-U™ Gene Knockout Cell Line Strategy

MAP3K2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAP3K2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAP3K2
Gene id 10746
Organism Homo sapiens
Official full symbol mitogen-activated protein kinase kinase kinase 2
Gene type protein-coding
Also known as MEKK2, MEKK2B
Summary The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process.
Genomic regions Chromosome 2
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAP3K2-203 ENST00000409947.5 10992 619aa Protein coding CCDS46404 A0A024RAH0 Q9Y2U5 - TSL:1, GENCODE basic, APPRIS P1,
MAP3K2-201 ENST00000344908.9 2135 619aa Protein coding CCDS46404 A0A024RAH0 Q9Y2U5 - TSL:1, GENCODE basic, APPRIS P1,
MAP3K2-202 ENST00000409179.2 689 47aa Protein coding - F8W6K3 - CDS 3' incomplete, TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAP3K2
This KO Strategy loading
Red Cotton™ Notes Gene MAP3K2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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