CRISPR-U™ Gene Knockout Cell Line
Strategy
STAG2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human STAG2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | STAG2 |
| Gene id | 10735 |
| Organism | Homo sapiens |
| Official full symbol | stromal antigen 2 |
| Gene type | protein-coding |
| Also known as | HPE13, MKMS, NEDXCF, SA-2, SA2, SCC3B, bA517O1.1 |
| Summary | The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| STAG2-203 | ENST00000371145.8 | 6179 | 1268aa | Protein coding | CCDS43990 | Q8N3U4-2 | NM_001042750.2 | TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| STAG2-205 | ENST00000371160.5 | 6045 | 1231aa | Protein coding | CCDS14607 | Q8N3U4-1 | - | TSL:1, GENCODE basic, APPRIS P3, |
| STAG2-204 | ENST00000371157.7 | 5991 | 1231aa | Protein coding | CCDS14607 | Q8N3U4-1 | - | TSL:1, GENCODE basic, APPRIS P3, |
| STAG2-201 | ENST00000218089.13 | 5218 | 1268aa | Protein coding | CCDS43990 | Q8N3U4-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| STAG2-202 | ENST00000371144.7 | 4281 | 1231aa | Protein coding | CCDS14607 | Q8N3U4-1 | - | TSL:1, GENCODE basic, APPRIS P3, |
| STAG2-211 | ENST00000455404.5 | 2089 | 548aa | Protein coding | - | B1AMT4 | - | CDS 3' incomplete, TSL:5, |
| STAG2-210 | ENST00000435215.5 | 798 | 207aa | Protein coding | - | B1AMT3 | - | CDS 3' incomplete, TSL:5, |
| STAG2-209 | ENST00000435103.5 | 742 | 154aa | Protein coding | - | B1AMT1 | - | CDS 3' incomplete, TSL:5, |
| STAG2-208 | ENST00000428941.5 | 678 | 169aa | Protein coding | - | B1AMT2 | - | CDS 3' incomplete, TSL:3, |
| STAG2-207 | ENST00000394478.1 | 676 | 115aa | Protein coding | - | B1AMT0 | - | CDS 3' incomplete, TSL:5, |
| STAG2-206 | ENST00000394477.5 | 385 | 70aa | Protein coding | - | B1AMS9 | - | CDS 3' incomplete, TSL:5, |
| STAG2-213 | ENST00000458700.5 | 296 | 40aa | Protein coding | - | B1AMS8 | - | CDS 3' incomplete, TSL:5, |
| STAG2-212 | ENST00000458176.5 | 845 | 58aa | Nonsense mediated decay | - | E7ERE6 | - | TSL:3, |
| STAG2-215 | ENST00000469481.1 | 989 | No protein | Processed transcript | - | - | - | TSL:3, |
| STAG2-214 | ENST00000466748.5 | 873 | No protein | Processed transcript | - | - | - | TSL:5, |
| STAG2-218 | ENST00000483575.5 | 864 | No protein | Processed transcript | - | - | - | TSL:5, |
| STAG2-216 | ENST00000471107.1 | 709 | No protein | Processed transcript | - | - | - | TSL:5, |
| STAG2-217 | ENST00000475602.1 | 356 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | STAG2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene STAG2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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