CRISPR-U™ Gene Knockout Cell Line Strategy

STAG2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human STAG2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol STAG2
Gene id 10735
Organism Homo sapiens
Official full symbol stromal antigen 2
Gene type protein-coding
Also known as HPE13, MKMS, NEDXCF, SA-2, SA2, SCC3B, bA517O1.1
Summary The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome X
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
STAG2-203 ENST00000371145.8 6179 1268aa Protein coding CCDS43990 Q8N3U4-2 NM_001042750.2 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
STAG2-205 ENST00000371160.5 6045 1231aa Protein coding CCDS14607 Q8N3U4-1 - TSL:1, GENCODE basic, APPRIS P3,
STAG2-204 ENST00000371157.7 5991 1231aa Protein coding CCDS14607 Q8N3U4-1 - TSL:1, GENCODE basic, APPRIS P3,
STAG2-201 ENST00000218089.13 5218 1268aa Protein coding CCDS43990 Q8N3U4-2 - TSL:1, GENCODE basic, APPRIS ALT1,
STAG2-202 ENST00000371144.7 4281 1231aa Protein coding CCDS14607 Q8N3U4-1 - TSL:1, GENCODE basic, APPRIS P3,
STAG2-211 ENST00000455404.5 2089 548aa Protein coding - B1AMT4 - CDS 3' incomplete, TSL:5,
STAG2-210 ENST00000435215.5 798 207aa Protein coding - B1AMT3 - CDS 3' incomplete, TSL:5,
STAG2-209 ENST00000435103.5 742 154aa Protein coding - B1AMT1 - CDS 3' incomplete, TSL:5,
STAG2-208 ENST00000428941.5 678 169aa Protein coding - B1AMT2 - CDS 3' incomplete, TSL:3,
STAG2-207 ENST00000394478.1 676 115aa Protein coding - B1AMT0 - CDS 3' incomplete, TSL:5,
STAG2-206 ENST00000394477.5 385 70aa Protein coding - B1AMS9 - CDS 3' incomplete, TSL:5,
STAG2-213 ENST00000458700.5 296 40aa Protein coding - B1AMS8 - CDS 3' incomplete, TSL:5,
STAG2-212 ENST00000458176.5 845 58aa Nonsense mediated decay - E7ERE6 - TSL:3,
STAG2-215 ENST00000469481.1 989 No protein Processed transcript - - - TSL:3,
STAG2-214 ENST00000466748.5 873 No protein Processed transcript - - - TSL:5,
STAG2-218 ENST00000483575.5 864 No protein Processed transcript - - - TSL:5,
STAG2-216 ENST00000471107.1 709 No protein Processed transcript - - - TSL:5,
STAG2-217 ENST00000475602.1 356 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene STAG2
This KO Strategy loading
Red Cotton™ Notes Gene STAG2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: