CRISPR-U™ Gene Knockout Cell Line
Strategy
PTGES3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTGES3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTGES3 |
| Gene id | 10728 |
| Organism | Homo sapiens |
| Official full symbol | prostaglandin E synthase 3 |
| Gene type | protein-coding |
| Also known as | P23, TEBP, cPGES |
| Summary | This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTGES3-207 | ENST00000614328.4 | 2077 | 164aa | Protein coding | CCDS73485 | A0A087WYT3 | - | TSL:3, GENCODE basic, |
| PTGES3-201 | ENST00000262033.11 | 1898 | 160aa | Protein coding | CCDS31836 | Q15185-1 | NM_006601.7 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PTGES3-202 | ENST00000414274.7 | 1547 | 130aa | Protein coding | CCDS61159 | Q15185-3 | - | TSL:2, GENCODE basic, |
| PTGES3-204 | ENST00000448157.6 | 1017 | 139aa | Protein coding | CCDS61160 | Q15185-4 | - | TSL:2, GENCODE basic, |
| PTGES3-203 | ENST00000436399.6 | 941 | 127aa | Protein coding | CCDS61158 | Q15185-2 | - | TSL:2, GENCODE basic, |
| PTGES3-205 | ENST00000456859.2 | 957 | 124aa | Protein coding | - | B4DDC6 | - | TSL:2, GENCODE basic, |
| PTGES3-206 | ENST00000537473.2 | 2939 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTGES3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTGES3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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