CRISPR-U™ Gene Knockout Cell Line Strategy

PTGES3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTGES3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTGES3
Gene id 10728
Organism Homo sapiens
Official full symbol prostaglandin E synthase 3
Gene type protein-coding
Also known as P23, TEBP, cPGES
Summary This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes.
Genomic regions Chromosome 12
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTGES3-207 ENST00000614328.4 2077 164aa Protein coding CCDS73485 A0A087WYT3 - TSL:3, GENCODE basic,
PTGES3-201 ENST00000262033.11 1898 160aa Protein coding CCDS31836 Q15185-1 NM_006601.7 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PTGES3-202 ENST00000414274.7 1547 130aa Protein coding CCDS61159 Q15185-3 - TSL:2, GENCODE basic,
PTGES3-204 ENST00000448157.6 1017 139aa Protein coding CCDS61160 Q15185-4 - TSL:2, GENCODE basic,
PTGES3-203 ENST00000436399.6 941 127aa Protein coding CCDS61158 Q15185-2 - TSL:2, GENCODE basic,
PTGES3-205 ENST00000456859.2 957 124aa Protein coding - B4DDC6 - TSL:2, GENCODE basic,
PTGES3-206 ENST00000537473.2 2939 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTGES3
This KO Strategy loading
Red Cotton™ Notes Gene PTGES3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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