CRISPR-U™ Gene Knockout Cell Line Strategy

CAMKK2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CAMKK2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CAMKK2
Gene id 10645
Organism Homo sapiens
Official full symbol calcium/calmodulin dependent protein kinase kinase 2
Gene type protein-coding
Also known as CAMKK, CAMKKB
Summary The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases.
Genomic regions Chromosome 12
Strategy Summary
This gene has 14 protein coding transcripts：
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CAMKK2-201 ENST00000324774.9 5598 588aa Protein coding CCDS9216 Q96RR4-1 - TSL:1, GENCODE basic, APPRIS P4,
CAMKK2-202 ENST00000337174.7 5304 541aa Protein coding CCDS9218 Q96RR4-3 - TSL:1, GENCODE basic, APPRIS ALT2,
CAMKK2-203 ENST00000347034.6 5218 545aa Protein coding CCDS9217 Q96RR4-4 - TSL:1, GENCODE basic,
CAMKK2-211 ENST00000538733.5 5175 498aa Protein coding CCDS9219 Q96RR4-5 - TSL:1, GENCODE basic,
CAMKK2-217 ENST00000652382.1 5155 588aa Protein coding CCDS9216 Q96RR4-1 - GENCODE basic, APPRIS P4,
CAMKK2-207 ENST00000404169.8 4905 588aa Protein coding CCDS9216 Q96RR4-1 NM_001270485.2 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CAMKK2-205 ENST00000392474.6 4453 556aa Protein coding CCDS58283 Q96RR4-7 - TSL:1, GENCODE basic,
CAMKK2-204 ENST00000392473.2 2967 533aa Protein coding CCDS44999 Q96RR4-2 - TSL:1, GENCODE basic,
CAMKK2-209 ENST00000446440.6 2587 490aa Protein coding CCDS53837 Q96RR4-6 - TSL:1, GENCODE basic,
CAMKK2-206 ENST00000402834.8 2051 588aa Protein coding CCDS9216 Q96RR4-1 - TSL:1, GENCODE basic, APPRIS P4,
CAMKK2-208 ENST00000412367.6 2006 541aa Protein coding CCDS9218 Q96RR4-3 - TSL:1, GENCODE basic, APPRIS ALT2,
CAMKK2-216 ENST00000545538.5 3797 343aa Protein coding - F5GZ00 - TSL:1, GENCODE basic,
CAMKK2-214 ENST00000543477.1 563 125aa Protein coding - F5H4I7 - CDS 3' incomplete, TSL:4,
CAMKK2-215 ENST00000544485.1 543 87aa Protein coding - F5H360 - CDS 3' incomplete, TSL:4,
CAMKK2-210 ENST00000535524.1 809 No protein Processed transcript - - - TSL:2,
CAMKK2-212 ENST00000539380.1 567 No protein Processed transcript - - - TSL:4,
CAMKK2-213 ENST00000542540.5 547 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CAMKK2
This KO Strategy loading
Red Cotton™ Notes Gene CAMKK2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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