CRISPR-U™ Gene Knockout Cell Line
Strategy
STAMBP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human STAMBP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | STAMBP |
| Gene id | 10617 |
| Organism | Homo sapiens |
| Official full symbol | STAM binding protein |
| Gene type | protein-coding |
| Also known as | AMSH, MICCAP |
| Summary | Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| STAMBP-202 | ENST00000394070.7 | 6746 | 424aa | Protein coding | CCDS1929 | O95630-1 | NM_213622.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| STAMBP-203 | ENST00000394073.5 | 6386 | 424aa | Protein coding | CCDS1929 | O95630-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| STAMBP-201 | ENST00000339566.7 | 6277 | 424aa | Protein coding | CCDS1929 | O95630-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| STAMBP-204 | ENST00000409707.5 | 1649 | 424aa | Protein coding | CCDS1929 | O95630-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| STAMBP-206 | ENST00000432295.6 | 1156 | 318aa | Protein coding | - | C9JK83 | - | CDS 3' incomplete, TSL:5, |
| STAMBP-211 | ENST00000536064.1 | 845 | 209aa | Protein coding | - | F5H5B9 | - | TSL:5, GENCODE basic, |
| STAMBP-205 | ENST00000424659.5 | 583 | 163aa | Protein coding | - | C9JEK5 | - | CDS 3' incomplete, TSL:3, |
| STAMBP-207 | ENST00000452725.5 | 560 | 137aa | Protein coding | - | C9JZ93 | - | CDS 3' incomplete, TSL:4, |
| STAMBP-209 | ENST00000486458.1 | 1012 | No protein | Processed transcript | - | - | - | TSL:3, |
| STAMBP-208 | ENST00000478946.1 | 955 | No protein | Processed transcript | - | - | - | TSL:3, |
| STAMBP-210 | ENST00000487811.1 | 695 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | STAMBP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene STAMBP had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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