CRISPR-U™ Gene Knockout Cell Line Strategy

NPC2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NPC2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NPC2
Gene id 10577
Organism Homo sapiens
Official full symbol NPC intracellular cholesterol transporter 2
Gene type protein-coding
Also known as EDDM1, HE1
Summary This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy.
Genomic regions Chromosome 14
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NPC2-209 ENST00000557510.5 1029 174aa Protein coding CCDS86413 G3V3E8 - TSL:1, GENCODE basic,
NPC2-207 ENST00000555619.6 821 151aa Protein coding CCDS32121 P61916-1 NM_006432.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
NPC2-203 ENST00000541064.5 1436 125aa Protein coding - P61916-2 - TSL:2, GENCODE basic,
NPC2-202 ENST00000434013.6 875 201aa Protein coding - E7EMS2 - TSL:2, GENCODE basic,
NPC2-201 ENST00000238633.6 796 148aa Protein coding - J3KMY5 - TSL:3, GENCODE basic, APPRIS ALT1,
NPC2-204 ENST00000553490.5 729 221aa Protein coding - G3V3D1 - CDS 3' incomplete, TSL:2,
NPC2-208 ENST00000556009.5 699 173aa Protein coding - H0YIZ1 - CDS 5' incomplete, TSL:5,
NPC2-206 ENST00000555592.1 469 122aa Protein coding - G3V2V8 - CDS 3' incomplete, TSL:5,
NPC2-205 ENST00000554482.1 583 53aa Nonsense mediated decay - H0YJE2 - CDS 5' incomplete, TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NPC2
This KO Strategy loading
Red Cotton™ Notes Gene NPC2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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