CRISPR-U™ Gene Knockout Cell Line
Strategy
NPC2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NPC2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NPC2 |
| Gene id | 10577 |
| Organism | Homo sapiens |
| Official full symbol | NPC intracellular cholesterol transporter 2 |
| Gene type | protein-coding |
| Also known as | EDDM1, HE1 |
| Summary | This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NPC2-209 | ENST00000557510.5 | 1029 | 174aa | Protein coding | CCDS86413 | G3V3E8 | - | TSL:1, GENCODE basic, |
| NPC2-207 | ENST00000555619.6 | 821 | 151aa | Protein coding | CCDS32121 | P61916-1 | NM_006432.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| NPC2-203 | ENST00000541064.5 | 1436 | 125aa | Protein coding | - | P61916-2 | - | TSL:2, GENCODE basic, |
| NPC2-202 | ENST00000434013.6 | 875 | 201aa | Protein coding | - | E7EMS2 | - | TSL:2, GENCODE basic, |
| NPC2-201 | ENST00000238633.6 | 796 | 148aa | Protein coding | - | J3KMY5 | - | TSL:3, GENCODE basic, APPRIS ALT1, |
| NPC2-204 | ENST00000553490.5 | 729 | 221aa | Protein coding | - | G3V3D1 | - | CDS 3' incomplete, TSL:2, |
| NPC2-208 | ENST00000556009.5 | 699 | 173aa | Protein coding | - | H0YIZ1 | - | CDS 5' incomplete, TSL:5, |
| NPC2-206 | ENST00000555592.1 | 469 | 122aa | Protein coding | - | G3V2V8 | - | CDS 3' incomplete, TSL:5, |
| NPC2-205 | ENST00000554482.1 | 583 | 53aa | Nonsense mediated decay | - | H0YJE2 | - | CDS 5' incomplete, TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NPC2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NPC2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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