CRISPR-U™ Gene Knockout Cell Line
Strategy
RABAC1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RABAC1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | RABAC1 |
| Gene id | 10567 |
| Organism | Homo sapiens |
| Official full symbol | Rab acceptor 1 |
| Gene type | protein-coding |
| Also known as | PRA1, PRAF1, YIP3 |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| RABAC1-201 | ENST00000222008.11 | 750 | 185aa | Protein coding | CCDS12593 | Q9UI14 | NM_006423.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| RABAC1-208 | ENST00000601078.5 | 728 | 91aa | Protein coding | - | M0QXU8 | - | TSL:3, GENCODE basic, |
| RABAC1-206 | ENST00000600292.5 | 653 | 149aa | Protein coding | - | M0R1H9 | - | CDS 5' incomplete, TSL:2, |
| RABAC1-210 | ENST00000601891.5 | 628 | 151aa | Protein coding | - | M0R3D4 | - | TSL:3, GENCODE basic, |
| RABAC1-207 | ENST00000601028.1 | 530 | 109aa | Protein coding | - | M0QXH1 | - | CDS 5' incomplete, TSL:2, |
| RABAC1-205 | ENST00000599219.1 | 799 | No protein | Retained intron | - | - | - | TSL:2, |
| RABAC1-209 | ENST00000601476.1 | 771 | No protein | Retained intron | - | - | - | TSL:2, |
| RABAC1-203 | ENST00000596171.1 | 759 | No protein | Retained intron | - | - | - | TSL:2, |
| RABAC1-202 | ENST00000595226.1 | 725 | No protein | Retained intron | - | - | - | TSL:2, |
| RABAC1-204 | ENST00000598057.1 | 662 | No protein | Retained intron | - | - | - | TSL:NA, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | RABAC1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene RABAC1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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