CRISPR-U™ Gene Knockout Cell Line
Strategy
IFI44 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFI44 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IFI44 |
| Gene id | 10561 |
| Organism | Homo sapiens |
| Official full symbol | interferon induced protein 44 |
| Gene type | protein-coding |
| Also known as | MTAP44, TLDC5, p44 |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IFI44-201 | ENST00000370747.9 | 1682 | 444aa | Protein coding | CCDS688 | Q8TCB0-1 | NM_006417.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IFI44-202 | ENST00000438486.1 | 686 | 195aa | Protein coding | - | C9JUM8 | - | CDS 3' incomplete, TSL:3, |
| IFI44-203 | ENST00000446486.1 | 497 | 76aa | Protein coding | - | H7C1F3 | - | CDS 5' incomplete, TSL:3, |
| IFI44-209 | ENST00000495254.5 | 1117 | No protein | Processed transcript | - | - | - | TSL:5, |
| IFI44-206 | ENST00000472152.5 | 917 | No protein | Processed transcript | - | - | - | TSL:5, |
| IFI44-205 | ENST00000470323.5 | 819 | No protein | Processed transcript | - | - | - | TSL:5, |
| IFI44-208 | ENST00000485662.1 | 611 | No protein | Processed transcript | - | - | - | TSL:3, |
| IFI44-207 | ENST00000476911.1 | 577 | No protein | Processed transcript | - | - | - | TSL:2, |
| IFI44-204 | ENST00000467790.5 | 1066 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IFI44 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IFI44 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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