CRISPR-U™ Gene Knockout Cell Line Strategy

IFI44 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IFI44 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IFI44
Gene id 10561
Organism Homo sapiens
Official full symbol interferon induced protein 44
Gene type protein-coding
Also known as MTAP44, TLDC5, p44
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IFI44-201 ENST00000370747.9 1682 444aa Protein coding CCDS688 Q8TCB0-1 NM_006417.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IFI44-202 ENST00000438486.1 686 195aa Protein coding - C9JUM8 - CDS 3' incomplete, TSL:3,
IFI44-203 ENST00000446486.1 497 76aa Protein coding - H7C1F3 - CDS 5' incomplete, TSL:3,
IFI44-209 ENST00000495254.5 1117 No protein Processed transcript - - - TSL:5,
IFI44-206 ENST00000472152.5 917 No protein Processed transcript - - - TSL:5,
IFI44-205 ENST00000470323.5 819 No protein Processed transcript - - - TSL:5,
IFI44-208 ENST00000485662.1 611 No protein Processed transcript - - - TSL:3,
IFI44-207 ENST00000476911.1 577 No protein Processed transcript - - - TSL:2,
IFI44-204 ENST00000467790.5 1066 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IFI44
This KO Strategy loading
Red Cotton™ Notes Gene IFI44 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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