CRISPR-U™ Gene Knockout Cell Line
Strategy
CIB2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CIB2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CIB2 |
| Gene id | 10518 |
| Organism | Homo sapiens |
| Official full symbol | calcium and integrin binding family member 2 |
| Gene type | protein-coding |
| Also known as | DFNB48, KIP2, USH1J |
| Summary | The protein encoded by this gene is similar to that of KIP/CIB, calcineurin B, and calmodulin. The encoded protein is a calcium-binding regulatory protein that interacts with DNA-dependent protein kinase catalytic subunits (DNA-PKcs), and it is involved in photoreceptor cell maintenance. Mutations in this gene cause deafness, autosomal recessive, 48 (DFNB48), and also Usher syndrome 1J (USH1J). Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CIB2-202 | ENST00000539011.5 | 1511 | 144aa | Protein coding | CCDS61722 | O75838-3 | - | TSL:1, GENCODE basic, |
| CIB2-201 | ENST00000258930.8 | 1499 | 187aa | Protein coding | CCDS10296 | O75838-1 | NM_006383.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CIB2-204 | ENST00000557846.5 | 934 | 138aa | Protein coding | CCDS61723 | O75838-4 | - | TSL:3, GENCODE basic, |
| CIB2-208 | ENST00000560618.5 | 804 | 167aa | Protein coding | - | H0YKC8 | - | TSL:2, GENCODE basic, |
| CIB2-207 | ENST00000559645.1 | 462 | 147aa | Protein coding | - | H0YKX8 | - | CDS 3' incomplete, TSL:3, |
| CIB2-206 | ENST00000559054.1 | 443 | 54aa | Protein coding | - | H0YLX3 | - | CDS 3' incomplete, TSL:2, |
| CIB2-209 | ENST00000561190.5 | 390 | 126aa | Protein coding | - | H0YLL7 | - | CDS 3' incomplete, TSL:3, |
| CIB2-210 | ENST00000643268.1 | 744 | 63aa | Nonsense mediated decay | - | A0A2R8Y4Y2 | - | CDS 5' incomplete, |
| CIB2-203 | ENST00000557818.1 | 669 | 48aa | Nonsense mediated decay | - | H0YML3 | - | CDS 5' incomplete, TSL:3, |
| CIB2-205 | ENST00000557917.5 | 535 | 92aa | Nonsense mediated decay | - | H0YND4 | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CIB2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CIB2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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