CRISPR-U™ Gene Knockout Cell Line Strategy

CIB2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CIB2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CIB2
Gene id 10518
Organism Homo sapiens
Official full symbol calcium and integrin binding family member 2
Gene type protein-coding
Also known as DFNB48, KIP2, USH1J
Summary The protein encoded by this gene is similar to that of KIP/CIB, calcineurin B, and calmodulin. The encoded protein is a calcium-binding regulatory protein that interacts with DNA-dependent protein kinase catalytic subunits (DNA-PKcs), and it is involved in photoreceptor cell maintenance. Mutations in this gene cause deafness, autosomal recessive, 48 (DFNB48), and also Usher syndrome 1J (USH1J). Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 15
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CIB2-202 ENST00000539011.5 1511 144aa Protein coding CCDS61722 O75838-3 - TSL:1, GENCODE basic,
CIB2-201 ENST00000258930.8 1499 187aa Protein coding CCDS10296 O75838-1 NM_006383.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CIB2-204 ENST00000557846.5 934 138aa Protein coding CCDS61723 O75838-4 - TSL:3, GENCODE basic,
CIB2-208 ENST00000560618.5 804 167aa Protein coding - H0YKC8 - TSL:2, GENCODE basic,
CIB2-207 ENST00000559645.1 462 147aa Protein coding - H0YKX8 - CDS 3' incomplete, TSL:3,
CIB2-206 ENST00000559054.1 443 54aa Protein coding - H0YLX3 - CDS 3' incomplete, TSL:2,
CIB2-209 ENST00000561190.5 390 126aa Protein coding - H0YLL7 - CDS 3' incomplete, TSL:3,
CIB2-210 ENST00000643268.1 744 63aa Nonsense mediated decay - A0A2R8Y4Y2 - CDS 5' incomplete,
CIB2-203 ENST00000557818.1 669 48aa Nonsense mediated decay - H0YML3 - CDS 5' incomplete, TSL:3,
CIB2-205 ENST00000557917.5 535 92aa Nonsense mediated decay - H0YND4 - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CIB2
This KO Strategy loading
Red Cotton™ Notes Gene CIB2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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