CRISPR-U™ Gene Knockout Cell Line
Strategy
CEBPB Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CEBPB Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CEBPB |
| Gene id | 1051 |
| Organism | Homo sapiens |
| Official full symbol | CCAAT enhancer binding protein beta |
| Gene type | protein-coding |
| Also known as | C/EBP-beta, IL6DBP, NF-IL6, TCF5 |
| Summary | This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CEBPB-201 | ENST00000303004.5 | 1839 | 345aa | Protein coding | CCDS13429 | P17676-1 | NM_005194.4 | TSL:NA, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CEBPB |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CEBPB had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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