CRISPR-U™ Gene Knockout Cell Line
Strategy
VTI1B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human VTI1B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | VTI1B |
| Gene id | 10490 |
| Organism | Homo sapiens |
| Official full symbol | vesicle transport through interaction with t-SNAREs 1B |
| Gene type | protein-coding |
| Also known as | VTI1, VTI1-LIKE, VTI1L, VTI2, v-SNARE, vti1-rp1 |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| VTI1B-204 | ENST00000554659.6 | 5142 | 232aa | Protein coding | CCDS9786 | Q9UEU0-1 | NM_006370.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| VTI1B-206 | ENST00000556461.1 | 576 | 103aa | Protein coding | - | H0YJL5 | - | CDS 5' incomplete, TSL:2, |
| VTI1B-203 | ENST00000554636.1 | 565 | 128aa | Protein coding | - | H0YJJ5 | - | CDS 5' incomplete, TSL:3, |
| VTI1B-201 | ENST00000216456.6 | 1103 | 44aa | Nonsense mediated decay | - | J3KMW2 | - | TSL:1, |
| VTI1B-205 | ENST00000555543.1 | 718 | 59aa | Nonsense mediated decay | - | G3V5I2 | - | TSL:2, |
| VTI1B-202 | ENST00000553619.1 | 574 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | VTI1B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene VTI1B had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: