CRISPR-U™ Gene Knockout Cell Line Strategy

VTI1B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human VTI1B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol VTI1B
Gene id 10490
Organism Homo sapiens
Official full symbol vesicle transport through interaction with t-SNAREs 1B
Gene type protein-coding
Also known as VTI1, VTI1-LIKE, VTI1L, VTI2, v-SNARE, vti1-rp1
Genomic regions Chromosome 14
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
VTI1B-204 ENST00000554659.6 5142 232aa Protein coding CCDS9786 Q9UEU0-1 NM_006370.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
VTI1B-206 ENST00000556461.1 576 103aa Protein coding - H0YJL5 - CDS 5' incomplete, TSL:2,
VTI1B-203 ENST00000554636.1 565 128aa Protein coding - H0YJJ5 - CDS 5' incomplete, TSL:3,
VTI1B-201 ENST00000216456.6 1103 44aa Nonsense mediated decay - J3KMW2 - TSL:1,
VTI1B-205 ENST00000555543.1 718 59aa Nonsense mediated decay - G3V5I2 - TSL:2,
VTI1B-202 ENST00000553619.1 574 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene VTI1B
This KO Strategy loading
Red Cotton™ Notes Gene VTI1B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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