CRISPR-U™ Gene Knockout Cell Line
Strategy
HMGN4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HMGN4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HMGN4 |
| Gene id | 10473 |
| Organism | Homo sapiens |
| Official full symbol | high mobility group nucleosomal binding domain 4 |
| Gene type | protein-coding |
| Also known as | HMG17L3, NHC |
| Summary | The protein encoded by this gene, a member of the HMGN protein family, is thought to reduce the compactness of the chromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HMGN4-201 | ENST00000377575.3 | 1943 | 90aa | Protein coding | CCDS4615 | A0A024R046 O00479 | NM_006353.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HMGN4-202 | ENST00000477243.1 | 113 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HMGN4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HMGN4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: