CRISPR-U™ Gene Knockout Cell Line Strategy

MERTK Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MERTK Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MERTK
Gene id 10461
Organism Homo sapiens
Official full symbol MER proto-oncogene, tyrosine kinase
Gene type protein-coding
Also known as MER, RP38, Tyro12, c-Eyk, c-mer
Summary This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP).
Genomic regions Chromosome 2
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MERTK-201 ENST00000295408.9 3826 999aa Protein coding CCDS2094 Q12866 NM_006343.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MERTK-203 ENST00000421804.6 3600 999aa Protein coding CCDS2094 Q12866 - TSL:1, GENCODE basic, APPRIS P1,
MERTK-202 ENST00000409780.5 3189 823aa Protein coding - E9PHX8 - TSL:5, GENCODE basic,
MERTK-207 ENST00000616902.4 3104 588aa Protein coding - A0A087WZQ5 - TSL:5, GENCODE basic,
MERTK-205 ENST00000449344.2 771 257aa Protein coding - H7C3L9 - CDS 5' and 3' incomplete, TSL:3,
MERTK-204 ENST00000439966.5 2972 96aa Nonsense mediated decay - E9PD22 - TSL:1,
MERTK-206 ENST00000473065.1 545 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MERTK
This KO Strategy loading
Red Cotton™ Notes Gene MERTK had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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