CRISPR-U™ Gene Knockout Cell Line
Strategy
MERTK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MERTK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MERTK |
| Gene id | 10461 |
| Organism | Homo sapiens |
| Official full symbol | MER proto-oncogene, tyrosine kinase |
| Gene type | protein-coding |
| Also known as | MER, RP38, Tyro12, c-Eyk, c-mer |
| Summary | This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MERTK-201 | ENST00000295408.9 | 3826 | 999aa | Protein coding | CCDS2094 | Q12866 | NM_006343.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MERTK-203 | ENST00000421804.6 | 3600 | 999aa | Protein coding | CCDS2094 | Q12866 | - | TSL:1, GENCODE basic, APPRIS P1, |
| MERTK-202 | ENST00000409780.5 | 3189 | 823aa | Protein coding | - | E9PHX8 | - | TSL:5, GENCODE basic, |
| MERTK-207 | ENST00000616902.4 | 3104 | 588aa | Protein coding | - | A0A087WZQ5 | - | TSL:5, GENCODE basic, |
| MERTK-205 | ENST00000449344.2 | 771 | 257aa | Protein coding | - | H7C3L9 | - | CDS 5' and 3' incomplete, TSL:3, |
| MERTK-204 | ENST00000439966.5 | 2972 | 96aa | Nonsense mediated decay | - | E9PD22 | - | TSL:1, |
| MERTK-206 | ENST00000473065.1 | 545 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MERTK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MERTK had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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