CRISPR-U™ Gene Knockout Cell Line
Strategy
TACC3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TACC3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TACC3 |
| Gene id | 10460 |
| Organism | Homo sapiens |
| Official full symbol | transforming acidic coiled-coil containing protein 3 |
| Gene type | protein-coding |
| Also known as | ERIC-1, ERIC1, Tacc4, maskin |
| Summary | This gene encodes a member of the transforming acidic colied-coil protein family. The encoded protein is a motor spindle protein that may play a role in stabilization of the mitotic spindle. This protein may also play a role in growth a differentiation of certain cancer cells. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TACC3-201 | ENST00000313288.9 | 2799 | 838aa | Protein coding | CCDS3352 | Q9Y6A5 | NM_006342.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| TACC3-214 | ENST00000651472.1 | 2764 | 838aa | Protein coding | CCDS3352 | Q9Y6A5 | - | GENCODE basic, APPRIS P2, |
| TACC3-216 | ENST00000652770.1 | 2826 | 834aa | Protein coding | - | A0A494BZT8 | - | GENCODE basic, APPRIS ALT2, |
| TACC3-211 | ENST00000612220.5 | 1732 | 478aa | Protein coding | - | Q8IX09 | - | TSL:5, APPRIS ALT2, |
| TACC3-203 | ENST00000458173.4 | 1093 | 272aa | Protein coding | - | E7EMT0 | - | CDS 3' incomplete, TSL:5, |
| TACC3-210 | ENST00000493975.5 | 825 | 230aa | Protein coding | - | C9JA91 | - | CDS 3' incomplete, TSL:3, |
| TACC3-206 | ENST00000470136.2 | 657 | 217aa | Protein coding | - | H0Y8F2 | - | CDS 5' incomplete, TSL:3, |
| TACC3-215 | ENST00000651817.1 | 2943 | 325aa | Nonsense mediated decay | - | A0A494C117 | - | CDS 5' incomplete, |
| TACC3-205 | ENST00000467746.6 | 792 | 63aa | Nonsense mediated decay | - | F8WC55 | - | TSL:3, |
| TACC3-213 | ENST00000651251.1 | 2788 | No protein | Processed transcript | - | - | - | - |
| TACC3-202 | ENST00000404054.3 | 2771 | No protein | Retained intron | - | - | - | TSL:NA, |
| TACC3-209 | ENST00000484651.5 | 2715 | No protein | Retained intron | - | - | - | TSL:2, |
| TACC3-212 | ENST00000650779.1 | 1567 | No protein | Retained intron | - | - | - | - |
| TACC3-207 | ENST00000470808.1 | 947 | No protein | Retained intron | - | - | - | TSL:2, |
| TACC3-204 | ENST00000466077.1 | 583 | No protein | Retained intron | - | - | - | TSL:2, |
| TACC3-208 | ENST00000484264.1 | 551 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TACC3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TACC3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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