CRISPR-U™ Gene Knockout Cell Line Strategy

TACC3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TACC3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TACC3
Gene id 10460
Organism Homo sapiens
Official full symbol transforming acidic coiled-coil containing protein 3
Gene type protein-coding
Also known as ERIC-1, ERIC1, Tacc4, maskin
Summary This gene encodes a member of the transforming acidic colied-coil protein family. The encoded protein is a motor spindle protein that may play a role in stabilization of the mitotic spindle. This protein may also play a role in growth a differentiation of certain cancer cells.
Genomic regions Chromosome 4
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TACC3-201 ENST00000313288.9 2799 838aa Protein coding CCDS3352 Q9Y6A5 NM_006342.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
TACC3-214 ENST00000651472.1 2764 838aa Protein coding CCDS3352 Q9Y6A5 - GENCODE basic, APPRIS P2,
TACC3-216 ENST00000652770.1 2826 834aa Protein coding - A0A494BZT8 - GENCODE basic, APPRIS ALT2,
TACC3-211 ENST00000612220.5 1732 478aa Protein coding - Q8IX09 - TSL:5, APPRIS ALT2,
TACC3-203 ENST00000458173.4 1093 272aa Protein coding - E7EMT0 - CDS 3' incomplete, TSL:5,
TACC3-210 ENST00000493975.5 825 230aa Protein coding - C9JA91 - CDS 3' incomplete, TSL:3,
TACC3-206 ENST00000470136.2 657 217aa Protein coding - H0Y8F2 - CDS 5' incomplete, TSL:3,
TACC3-215 ENST00000651817.1 2943 325aa Nonsense mediated decay - A0A494C117 - CDS 5' incomplete,
TACC3-205 ENST00000467746.6 792 63aa Nonsense mediated decay - F8WC55 - TSL:3,
TACC3-213 ENST00000651251.1 2788 No protein Processed transcript - - - -
TACC3-202 ENST00000404054.3 2771 No protein Retained intron - - - TSL:NA,
TACC3-209 ENST00000484651.5 2715 No protein Retained intron - - - TSL:2,
TACC3-212 ENST00000650779.1 1567 No protein Retained intron - - - -
TACC3-207 ENST00000470808.1 947 No protein Retained intron - - - TSL:2,
TACC3-204 ENST00000466077.1 583 No protein Retained intron - - - TSL:2,
TACC3-208 ENST00000484264.1 551 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TACC3
This KO Strategy loading
Red Cotton™ Notes Gene TACC3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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