CRISPR-U™ Gene Knockout Cell Line
Strategy
ECI2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ECI2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ECI2 |
| Gene id | 10455 |
| Organism | Homo sapiens |
| Official full symbol | enoyl-CoA delta isomerase 2 |
| Gene type | protein-coding |
| Also known as | ACBD2, DRS-1, DRS1, HCA88, PECI, dJ1013A10.3 |
| Summary | This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ECI2-207 | ENST00000465828.5 | 1548 | 364aa | Protein coding | CCDS4490 | A0A0C4DGA2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| ECI2-204 | ENST00000380125.6 | 1405 | 364aa | Protein coding | CCDS4490 | A0A0C4DGA2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ECI2-201 | ENST00000361538.6 | 1380 | 364aa | Protein coding | CCDS4490 | A0A0C4DGA2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ECI2-202 | ENST00000380118.8 | 1368 | 394aa | Protein coding | CCDS43420 | O75521-1 | NM_206836.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| ECI2-210 | ENST00000495548.1 | 814 | 220aa | Protein coding | - | C9JB63 | - | CDS 3' incomplete, TSL:5, |
| ECI2-203 | ENST00000380120.6 | 1469 | 152aa | Nonsense mediated decay | - | F8W6J1 | - | TSL:2, |
| ECI2-211 | ENST00000496241.6 | 1451 | 140aa | Nonsense mediated decay | - | F8WAW4 | - | TSL:1, |
| ECI2-208 | ENST00000478266.6 | 1133 | 197aa | Nonsense mediated decay | - | F1LLU7 | - | TSL:5, |
| ECI2-206 | ENST00000464583.5 | 3870 | No protein | Retained intron | - | - | - | TSL:2, |
| ECI2-205 | ENST00000464057.5 | 2122 | No protein | Retained intron | - | - | - | TSL:2, |
| ECI2-209 | ENST00000489086.1 | 558 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ECI2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ECI2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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