CRISPR-U™ Gene Knockout Cell Line Strategy

ECI2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ECI2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ECI2
Gene id 10455
Organism Homo sapiens
Official full symbol enoyl-CoA delta isomerase 2
Gene type protein-coding
Also known as ACBD2, DRS-1, DRS1, HCA88, PECI, dJ1013A10.3
Summary This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described.
Genomic regions Chromosome 6
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ECI2-207 ENST00000465828.5 1548 364aa Protein coding CCDS4490 A0A0C4DGA2 - TSL:5, GENCODE basic, APPRIS ALT2,
ECI2-204 ENST00000380125.6 1405 364aa Protein coding CCDS4490 A0A0C4DGA2 - TSL:1, GENCODE basic, APPRIS ALT2,
ECI2-201 ENST00000361538.6 1380 364aa Protein coding CCDS4490 A0A0C4DGA2 - TSL:1, GENCODE basic, APPRIS ALT2,
ECI2-202 ENST00000380118.8 1368 394aa Protein coding CCDS43420 O75521-1 NM_206836.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
ECI2-210 ENST00000495548.1 814 220aa Protein coding - C9JB63 - CDS 3' incomplete, TSL:5,
ECI2-203 ENST00000380120.6 1469 152aa Nonsense mediated decay - F8W6J1 - TSL:2,
ECI2-211 ENST00000496241.6 1451 140aa Nonsense mediated decay - F8WAW4 - TSL:1,
ECI2-208 ENST00000478266.6 1133 197aa Nonsense mediated decay - F1LLU7 - TSL:5,
ECI2-206 ENST00000464583.5 3870 No protein Retained intron - - - TSL:2,
ECI2-205 ENST00000464057.5 2122 No protein Retained intron - - - TSL:2,
ECI2-209 ENST00000489086.1 558 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ECI2
This KO Strategy loading
Red Cotton™ Notes Gene ECI2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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