CRISPR-U™ Gene Knockout Cell Line
Strategy
FAM3C Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FAM3C Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FAM3C |
| Gene id | 10447 |
| Organism | Homo sapiens |
| Official full symbol | FAM3 metabolism regulating signaling molecule C |
| Gene type | protein-coding |
| Also known as | GS3786, ILEI |
| Summary | This gene is a member of the family with sequence similarity 3 (FAM3) family and encodes a secreted protein with a GG domain. A change in expression of this protein has been noted in pancreatic cancer-derived cells. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FAM3C-201 | ENST00000359943.8 | 2455 | 227aa | Protein coding | CCDS5782 | Q92520 | NM_014888.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| FAM3C-203 | ENST00000426156.1 | 662 | 109aa | Protein coding | - | C9JP35 | - | CDS 3' incomplete, TSL:5, |
| FAM3C-202 | ENST00000412653.5 | 561 | 151aa | Protein coding | - | C9JMN4 | - | CDS 3' incomplete, TSL:4, |
| FAM3C-205 | ENST00000497622.1 | 666 | No protein | Retained intron | - | - | - | TSL:2, |
| FAM3C-204 | ENST00000474082.1 | 567 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FAM3C |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FAM3C had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: