CRISPR-U™ Gene Knockout Cell Line Strategy

CD2BP2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CD2BP2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CD2BP2
Gene id 10421
Organism Homo sapiens
Official full symbol CD2 cytoplasmic tail binding protein 2
Gene type protein-coding
Also known as FWP010, LIN1, PPP1R59, Snu40, U5-52K
Summary This gene encodes a bi-functional protein. In the cytoplasm, the encoded protein binds the cytoplasmic tail of human surface antigen CD2 via its C-terminal GYF domain, and regulate CD2-triggered T lymphocyte activation. In the nucleus, this protein is a component of the U5 small nuclear ribonucleoprotein complex and is involved in RNA splicing. A pseudogene has been identified on chromosome 7. Alternative splicing results in multiple transcript variants but their biological validity has not been determined.
Genomic regions Chromosome 16
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CD2BP2-201 ENST00000305596.8 3361 341aa Protein coding CCDS10675 A0A024QZC1 O95400 NM_006110.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CD2BP2-203 ENST00000569466.1 1417 341aa Protein coding CCDS10675 A0A024QZC1 O95400 - TSL:1, GENCODE basic, APPRIS P1,
CD2BP2-202 ENST00000564525.1 541 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CD2BP2
This KO Strategy loading
Red Cotton™ Notes Gene CD2BP2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
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