CRISPR-U™ Gene Knockout Cell Line
Strategy
IFITM3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFITM3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IFITM3 |
| Gene id | 10410 |
| Organism | Homo sapiens |
| Official full symbol | interferon induced transmembrane protein 3 |
| Gene type | protein-coding |
| Also known as | 1-8U, DSPA2b, IP15 |
| Summary | The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 15 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IFITM3-201 | ENST00000399808.5 | 611 | 133aa | Protein coding | CCDS41585 | Q01628 | NM_021034.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| IFITM3-217 | ENST00000680622.1 | 884 | 62aa | Protein coding | - | - | - | CDS 3' incomplete, |
| IFITM3-204 | ENST00000602735.2 | 713 | 112aa | Protein coding | - | E9PS44 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| IFITM3-215 | ENST00000680209.1 | 651 | 97aa | Protein coding | - | - | - | GENCODE basic, |
| IFITM3-224 | ENST00000681198.1 | 591 | 92aa | Protein coding | - | - | - | GENCODE basic, |
| IFITM3-202 | ENST00000526811.4 | 543 | 112aa | Protein coding | - | E9PS44 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| IFITM3-225 | ENST00000681304.1 | 517 | 42aa | Protein coding | - | - | - | GENCODE basic, |
| IFITM3-227 | ENST00000681840.1 | 505 | 38aa | Protein coding | - | - | - | GENCODE basic, |
| IFITM3-223 | ENST00000681184.1 | 277 | 62aa | Protein coding | - | - | - | CDS 3' incomplete, |
| IFITM3-208 | ENST00000679433.1 | 238 | 62aa | Protein coding | - | - | - | CDS 3' incomplete, |
| IFITM3-209 | ENST00000679441.1 | 238 | 62aa | Protein coding | - | - | - | CDS 3' incomplete, |
| IFITM3-211 | ENST00000679792.1 | 238 | 62aa | Protein coding | - | - | - | CDS 3' incomplete, |
| IFITM3-214 | ENST00000680023.1 | 238 | 62aa | Protein coding | - | - | - | CDS 3' incomplete, |
| IFITM3-219 | ENST00000680720.1 | 238 | 62aa | Protein coding | - | - | - | CDS 3' incomplete, |
| IFITM3-226 | ENST00000681748.1 | 238 | 62aa | Protein coding | - | - | - | CDS 3' incomplete, |
| IFITM3-207 | ENST00000679382.1 | 587 | 186aa | Non stop decay | - | - | - | CDS 3' incomplete, |
| IFITM3-203 | ENST00000531688.2 | 725 | 98aa | Nonsense mediated decay | - | R4GNG9 | - | TSL:3, |
| IFITM3-205 | ENST00000602809.1 | 386 | No protein | Processed transcript | - | - | - | TSL:5, |
| IFITM3-212 | ENST00000679793.1 | 345 | No protein | Processed transcript | - | - | - | - |
| IFITM3-220 | ENST00000680920.1 | 345 | No protein | Processed transcript | - | - | - | - |
| IFITM3-218 | ENST00000680716.1 | 140 | No protein | Processed transcript | - | - | - | - |
| IFITM3-216 | ENST00000680372.1 | 94 | No protein | Processed transcript | - | - | - | - |
| IFITM3-210 | ENST00000679746.1 | 60 | No protein | Processed transcript | - | - | - | - |
| IFITM3-213 | ENST00000679830.1 | 60 | No protein | Processed transcript | - | - | - | - |
| IFITM3-222 | ENST00000681048.1 | 60 | No protein | Processed transcript | - | - | - | - |
| IFITM3-221 | ENST00000680932.1 | 1044 | No protein | Retained intron | - | - | - | - |
| IFITM3-206 | ENST00000602949.1 | 592 | No protein | Retained intron | - | - | - | TSL:NA, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IFITM3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IFITM3 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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